Tim-4基因rs7700944位点多态性与宁夏回族人群RA的关联性分析

Correlation between rs7700944 SNP of Tim-4 gene and rheumatoid arthritis in Ningxia Hui ethnicity

目的对宁夏回族人群Tim-4基因rs7700944位点多态性与类风湿关节炎(RA)的关联性进行分析,旨在为RA的早期预防提供理论依据。方法采用限制性片段长度多态性(PCR-RFLP)方法,对宁夏回族210例RA患者(RA组)和198名健康个体(对照组)Tim-4基因rs7700944位点进行基因型检测及分析。结果在对照组及RA组间,基因型频率(χ2=29.051)及等位基因频率(χ2=14.106)的分布差异均有统计学意义(P<0.01);等位基因A的频率在对照组中显著高于RA组,OR(95%CI)为0.549(0.400～0.752);而等位基因G的频率在对照组中显著低于RA组,OR(95%CI)为1.823(1.330～2.498)(P<0.01)。结论 Tim-4基因rs7700944位点多态性与宁夏回族人群RA的发生具有关联性,其中等位基因G为宁夏回族人群易患RA的危险因子,而等位基因A为保护因子。

Objective To investigate the correlation between rs7700944 SNP of Tim-4 gene and rheumatoid arthritis（RA） in Ningxia Hui ethnicity to provide theoretic basis for taking early prevention of RA.Methods The polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP） genotyping method was carried out to examine SNP at rs7700944 site in Tim-4 gene,including 210 Ningxia Hui RA patients and 198 ethnically matched non-arthritis（non-RA） controls.Results The statistical analysis demonstrated the genotype and allele frequencies at this polymorphic site for RA patients were significantly different from non-RA cohorts in the Ningxia Hui population（χ2=29.051 and 14.106,P〈0.01）.A allele was more frequent in the non-RA Hui individuals than that without RA,and OR（95%CI） value was 0.549（0.400-0.752）.In contrast,G allele was less common in Hui non-RA individuals than that in RA patients,and OR（95%CI） value was 1.823（1.330-2.498）（P〈0.01）.Conclusion rs7700944 polymorphism of Tim-4 gene may be strongly associated with RA susceptibility in Ningxia Hui ethnicity.G allele is the risk factor of RA and A allele is the protective factor of RA in Ningxia Hui ethnicity.