红外光谱法研究静磁场对大鼠脑组织的影响

FTIR study of rat brain exposure to static magnetic field

电气工业的发展使得人们生存的环境中充满了各类频率的电磁污染,为了探讨静磁场可能产生的生物学效应及其发生机制,从实验上研究了0.17 T静磁场曝露作用30天后对于大鼠脑组织红外光谱特征的影响,实验结果显示实验组1549 cm-1附近的酰胺Ⅱ带吸收峰有较为明显的红移现象,在1635 cm-1处为蛋白质酰胺Ⅰ带峰位置有较大的差异,另外,利用二阶导数谱分析了实验组与对照组在分子结构上存在的差异。这些变化可以反映脑组织在静磁场曝露作用前后组成成分的变化,说明傅里叶变换红外光谱方法是研究电磁场不同生物效应的发生机制的一种有效手段。

Development of electrical industry makes many kinds of electromagnetic pollution in living environment.In order to explore the biological effects and mechanisms of the static magnetic field（SMF）,Fourier transform infrared spectroscopy（FTIR） analysis method is used to study changes in brain tissue of rat brain exposed to SMF（0.17 T） for 30 days in the experiment.Experiment shows that the absorption peak of amide Ⅱ near 1549 cm-1 has a significant red-shift,peak position of amide Ⅰ near 1635 cm-1 has a great difference.Differences of molecular structure between the experiment group and the sample group are also analyzed via the second derivative spectra.These results can reflect changes of function and composition in rat brain which is exposed to SMF.Results of this study also indicate that the method of FTIR is an effective means to study the biological effects of electromagnetic field.