帕金森病患者血清中铜、铁、锰、锌和维生素E、维生素B12的测定

Study on the Relationship between Serum Cu、Fe、Mn、Zn、Vitamin E、Vitamin B12 Level and Parkinson's Disease

目的探讨帕金森病患者血清中铜、铁、锰、锌和维生素E、维生素B12与帕金森病的关系。方法采用1∶1配对的病例对照研究方法,收集最近4年内新发帕金森病病例40例和年龄(±3岁)、性别、职业、文化程度匹配的对照者40例,采用经石墨炉原子吸收法测定帕金森病患者和对照组血清中微量元素锰含量;帕金森病患者和对照组血清中微量元素铜、铁、锌的含量则用电感耦合等离子体发射光谱ICP直接测定法,用高效液相色谱来测定帕金森病患者和对照组血清中维生素E、维生素B12的含量,并进行配对t检验分析。结果帕金森病患者血清中锰、铁含量高于对照组(P<0.05),而两组间铜、锌、维生素E、维生素B12含量则无明显差异(P>0.05)。结论血清微量元素锰、铁可能是帕金森病发病的重要影响因素之一。

Objective To study the relationship between serum Cu.Fe.Mn.Zn.Vitamin E.Vitamin B12 level and Parkinson＇s disease（PD）. Methods We enrolled 40 cases of PD patients in last 4 years and 40 cases of age-, sex-, and race-, frequency-matched controls in a case control study. Serum concentration of Mn was measured with graphite atomic absorption, Cu. Fe. Zn with Inductively Coupled Plasma Mass Spectrometry （ICP）, and the Vitamin E.Vitamin B12 level with High-performance liquid chromatography（HPLC）. Results Concentration of Fe.Mn were significantly higher in PD patients than that in the controls（P〈0. 05）, while the Cu.Zn.Vitamin E. Vitamin B12 concentration in PD patients was not significantly different from that in the controls（P〉0. 05）. Conclusions The pathogenesis and development of PD might be related to some trace met- als especially Fe and Mn.