柠檬酸对小鼠睾丸组织抗氧化酶活性的影响

Effects of Citric Acid on Activities of Antioxidant Enzymes of Testis in Mice

为研究柠檬酸对小鼠睾丸组织抗氧化酶活性的影响,将40只健康昆明系小鼠随机分为对照组(生理盐水)、柠檬酸低(180mg/kg)、中(270mg/kg)、高(360mg/kg)剂量组,各组小鼠每周腹腔注射相应剂量试剂0.2mL,连续注射3周,第3次注射后的第7天时颈椎脱臼处死小鼠,取其睾丸组织制作匀浆液检测过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)、总一氧化氮合酶(T-NOS)活性及丙二醛(MDA)含量。结果表明,与对照组相比,中、高柠檬酸剂量组小鼠睾丸组织SOD活性显著降低(P<0.05);柠檬酸处理组GSH-Px活性、MDA含量均显著下降(P<0.05);随着柠檬酸剂量的增加,LDH、NOS活性分别呈下降、上升趋势。此结果提示,一定剂量的柠檬酸可以降低小鼠睾丸组织SOD、GSH-Px活性,但可能通过NO途径减少睾丸细胞膜脂质过氧化,提高细胞膜的稳定性。

To study effects of citric acid on the activities of antioxidant enzymes of testis in mice, 40 healthy Kun- ming mice were randomly divided into control group （0. 9% saline）, low dose group （180 mg/kg）, middle dose group （270 mg/kg） and high dose group （360 mg/kg）. All the mice were received an injection of 0.2 mL corre- sponding reagent each week for 3 weeks. After the third injection of seventh day, all the mice were killed by cervi- cal dislocation, superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, lactate dehydrogenase （LDH）, total nitric oxide synthase （T-NOS） activities and malondialdehyde （MDA） contents were detected with the testic- ular tissue homogenate. The results showed that the SOD activity was significantly decreased in middle and high dose groups （P〈0.05） compared with the control group, the activity of GSH-Px and MDA content were signifi- cantly degraded （P〈0.05） in the citric acid treatment groups, with citric acid dose increased, the LDH and NOS activities were showed decreasing, rising trend respectively. The results suggested that a certain dose of citric acid can degrade the SOD, GSH-Px activities of testis in mice, but the reduction of lipid peroxidation and the stability in testicular cell membrane may be via NO pathway.