小儿实体瘤手术前后细胞因子sIL-2R、TNFα的表达及意义

Expression and significance of sIL-2R and TNFα in children with solid tumors

目的 探讨小儿良、恶性实体瘤sIL 2R、TNFα的表达差异及其术后变化规律。方法 选择 30例恶性实体瘤、15例良性实体瘤和 15例健康儿童 ,分别检测术前 1d ,术后 2d、3d、7d的血清sIL 2R(ELISA双抗体夹心法 )和TNFα(RIA法 )含量。结果 sIL 2R和TNFα无论在恶性实体瘤组(分别为 998.37u/ml和 2 .133pg/ml)还是良性组 (分别为5 0 6 .40u/ml和 0 .983pg/ml) ,均较健康对照组 (分别为 342 .93u/ml和 0 .76 7pg/ml)显著增高 (P <0 .0 5 ) ,而恶性组又显著高于良性组水平 (P <0 .0 5 ) ,术后 1周内 ,sIL 2R在恶性组呈上升趋势 ,良性组则逐渐下降 ;TNFα在恶性组和良性组均于术后呈下降势态 ,而于 7d上升至术前水平。结论 sIL 2R和TNFα的升高 ,可作为实体瘤发生的生物学标志 ,并可作为鉴别良、恶性实体瘤的补充标志 ,术后动态定量观察有助于了解机体免疫状况和预后的判断。

Objective To evaluate the levels of serum soluble interleukin-2 receptor (sIL-2R) and tumor necrosis factor-α (TNFα) in pediatric benign and malignant solid tumors before and after operation. Methods Serum sIL-2R(ELISA) and TNFα (RIA) were determined prior to and 2,3,7 days after surgical removal of 45 cases with benign (n=30) and malignant solid tumors(n=15).And 15 normal children served as controls. Results The levels of sIL-2R and TNFα in children with benign or malignant solid tumor were significantly higher than that of the controls (P<0.05) and the level in malignant group was significantly higher than that in benign group. The level of sIL-2R tended to increase one-week postoperatively in malignant group and decrease in benign one. The levels of TNFα in both malignant and benign groups exhibited a decreasing trend on day 2 to 3 postoperatively, but returned to pre-operative level on the 7th day postoperatively. Conclusions The level of sIL-2R or TNFα can be used as a solid tumor marker and as a supplementary marker for differentiating malignant tumors from benign ones.