RAN基因多态性与广西人群乙型肝炎病毒相关肝细胞癌的遗传关联研究

Association between RAN polymorphisms and susceptibility to hepatitis B related hepatocellular carcinoma in Guangxi

目的探讨肿瘤相关基因RAN多态性与广西人群乙型肝炎病毒(HBV)相关肝细胞癌(HCC)遗传易感性的相关性。方法选取位于RAN启动子区rs7132224和3'非翻译区rs14035两个单核苷酸多态性(SNP)为遗传标记;采用SNPstream对340例HBV相关的HCC和361例对照个体进行基因分型;Logistic回归分析计算OR和95%CI。结果①rs7132224、rs14035和HBV相关HCC遗传易感性相关性评价结果分别为OR=1.43,95%CI=1.04～1.98,P=0.02;OR=1.43,95%CI=0.57～4.11,P=0.40;②对人群进行性别、年龄、吸烟、饮酒状态、一级生物学亲属HCC家族史分层分析,发现rs7132224、rs14035和各亚人群HBV HCC发生均无明显相关性。结论 rs7132224和HBV相关HCC遗传易感性显著相关,而rs14035与HBV相关HCC遗传易感性无显著相关性。rs7132224、rs14035与性别、年龄、饮酒状态、吸烟状态、一级生物学亲属HCC家族史在HBV相关HCC发生风险中无交互作用。

Objective To investigate the association between the single nucleotide polymorphisms（SNPs） in RAN gene and susceptibility of HBV-related hepatocellular carcinoma （HCC） in Guangxi population. Methods Two SNPs were selected in which rs7132224 was located in promoter region and rs14035 was located in 3＇ untranslated region. 340 cases were genotyped by SNPstream with 361 control individuals. Logistic regression analysis was performed to calculate odds ratio （OR） and 95% confidence interval （95% CI）. Results （1） The evaluation results of genetic susceptibility between rs7132224, rs14035 and HBV-related HCC are as follows： OR = 1.43, 95% CI = 1.04 - 1.98, P = 0. 02; rs14035, OR = 1.43, 95% CI = 0. 57 - 4. 11. （2） There were no significant correlation between rs7132224 or rs14035 and HBV-related HCC risk, When the analyses were stratified by sex, age, status of smoking drinking and the first degree family history of HCC. Conclusion Significant association with the risk of HBV-related HCC was observed with RAN rs7132224 but not with RAN rs14035. There is no interaction between rs7132224 or rs14035 and sex, age,status of smoking, drinking, as well as the first degree family history oeeured in HBV related HCC risk.