3种粉尘对兔肺泡巨噬细胞合成NO及NOS活性的影响

Quartz and Chrysotile Up regulate Nitric Oxide and Nitric Oxide Synthase Activity in Rabbit Alveolar Macrophages

为探讨一氧化氮 (NO)和一氧化氮合酶 (NOS)在粉尘致病中的作用 ,利用改良Myvirk法收集兔肺泡巨噬细胞 (AM)进行体外培养 ,采用硝酸还原酶法测定二氧化钛、石英尘和新康温石棉处理AM后培养上清液中亚硝酸根 /硝酸根 (NO-2 /NO-3 )的含量 (NO的静态氧化形式 ) ,同时利用酶 底物反应形成有色物质测定AM的细胞裂解液中NOS的酶活性。结果显示 ,随粉尘浓度的增加 :(1)温石棉组和石英组的AM死亡率增高 ,且温石棉组 >石英组 >二氧化钛组 ;(2 )温石棉组和石英组NO-2 /NO-3 的含量增加 ,呈剂量反应关系 (P <0 0 5 ) ,在高剂量组 ,两组NO均高于二氧化钛组 ,温石棉组和石英组之间无显著性差别 (P >0 0 5 ) ;(3)石英和温石棉均使NOS活性随剂量增加而升高 ,但高剂量石棉组的NOS活性降低 ,这可能是AM死亡率增高所致 ;(4 )石英和温石棉介导的NO和NOS活性变化具有一致性。

In order to explore the role of nitric oxide(NO) and nitric oxide synthase(NOS) in dust (quartz and asbestos) caused diseases,rabbit avleolar macrophages collected by Myvirk method were cultured in vitro.NO - 2/NO - 3,the stable oxide formation of NO,in the culturing supernatants of TiO 2-,quartz- and chrysotile- treated AMs was measured by nitrate reductase reaction method,and NOS activity was measured with color reaction method(enzyme substant reaction).The results showed that with the increase of dust concentration,(1)AM mortality increased in both quartz and chrysotile group,the effect of different dust was different:chrysotile>quartz>TiO 2.(2)In both quartz and chrysotile groups,NO - 2/NO - 3 increaed with dose response relationship(P<0 05);at the higher dose group,NO in both groups was higher than that of TiO 2 group respectively,but there was no difference in quartz and chrysotile group(P>0 05).(3) NOS activity elevated in quartz and chrysotile groups,but in 100,200μg/ml chrysotile group,the NOS activity decreased,which was maybe result from AM mortality increasing.(4)The change of NO was agreement with the change of NOS activity in quartz- and chrysotile exposed AMs.It was suggested that NO and NOS were maybe potential role in quartz- and chrysotile related diseases.