蒺藜苜蓿MtROP5启动子与GUS基因融合构建的稳定转化及其表达特性

Stable Transformation and Tissue Expression of Medicago truncatula Gaertn. Directed by MtROP5 Gene Promoter

本文以3～4周大小的蒺藜苜蓿R108．1的叶片为外植体，通过根癌农杆菌介导的遗传转化系统将MtROP5启动子与GUS报告基因的融合构建导入蒺藜苜蓿中，该转化系统经体胚发生产生转化的再生植株。利用PCR扩增对转化植株进行初步鉴定，并通过GUS4J6学染色法分析MtROP5启动子的组织表达特性。结果表~）]MtROP5启动子主要在蒺藜苜蓿根、花、果荚、叶片和种子中表达。

In this study, we used the leaves of 3 to 4-week-old plants of Medicago truncatula R108-1 as explants, and introduced the construct harboring MtROP5 promoter fused to reporter GUS into Medicago truncatula through Agrobacterium-mediated transformation. The regenerated plants were obtained via somatic embryogenesis. We first identified the regenerated plants by PCR, and further analyzed tissue expression of MtROP5 promoter using GUS histochemical assay. The results showed that MtROP5 promoter predominantly expressed in roots, flowers, pods, leaves and seeds.