嘉庚蛸精子形成过程中KIFC1类似物与NUP62分布的免疫荧光分析

Immunofluorescence Analysis of the Distribution of KIFC1-like Protein and NUP62 during Spermiogenesis in Octopus tankahkeei

精子发生（spermatogenesis）是受基因调控的复杂的发育过程,精子形成不同阶段生精细胞内基因特异性表达导致顶体的发生、精核形态的建成及尾部的形成,精细胞内特有的微管套（manchette）和活动于微管套上的各种分子马达（molecularmotor）在上述各结构形成中发挥重要作用。

Immunofluorescence and confocal microscopy were employed to investigate the localization pattern of a KIFCl-like protein and NUP62 as well as their relationship during spermiogenesis in Octopus tankah- keei. In early spermatid, the nucleus was round or oval, expression of the KIFC l-like protein was not obvious, and NUP62 was abundantly located in the nuclear membrane. In intermediate spermatid, the nucleus became spindleshaped, the KIFCl-like protein was apparently expressed and located around the nucleus, and NUP62 was mainly located in the nuclear membrane. In late spermatid, the nucleus resembled a long spindle, the KIFCl-like protein was concentrated in proximity to the nuclear periphery and also located within the nucleus, and NUP62 was distributed in the nuclear membrane and within the nucleus. In immature sperm, the nucleus was cylindrical, distribution of the KIFC l-like protein and NUP62 was similar to that in late spermatid, and the KIFC l-like protein was enriched at two ends of the cell. In mature sperm, the nucleus became long rod-shaped, expression of the KIFC l-like protein was not detected, and expression of NUP62 was maintained at a high level. The results indicated that the KIFC l-like protein was probably involved in the biogenesis of acrosome and through its interaction with NUP62 or other mediator, associated with some macromolecular assembly in the nuclear membrane, thus providing the nucleus with direct shaping force or influencing the shaping process via indirect regulation.