高效毛细管电泳分离测定头孢克罗血浆浓度

Separation and Determination of Cefaclor in Human Plasma by High Performance Capillary Electrophoresis

采用乙腈直接沉淀血浆蛋白 ,以头孢拉定为内标 ,建立了适合人血浆中头孢克罗分离测定的高效毛细管电泳方法。在 4 8cm× 50μm未涂层石英毛细管柱上 ,选择 50 mmol/L磷酸二氢钠 -1 2 .5mmol/L硼砂( p H8.0 8)为运行缓冲液 ,操作电压 2 0 k V( +)→ ( -) ,压力进样 1 2 psi×s,柱上紫外 2 65nm检测 ,头孢克罗和内标头孢拉定在 6.5min内获得了理想的分离 ,内源性物质不干扰高效毛细管电泳定量分析。血药浓度在 0 .2 5～ 2 5.0μg/ml范围内线性关系良好 ( r=0 .9993 ,n=5) ,绝对回收率大于 90 .5% ,日内及日间精密度均小于 9.5% ,并成功地应用于健康志愿者单剂量口服头孢克罗普通胶囊后血药浓度的动态分析。

A high performance capillary electrophoretic(HPCE)method was established for the separation and determination of cefaclor in human plasma with cefradine as the internal standard and plasma sample was simply deproteinized with acetonitrile.Based on the mode of capillary zone electrophoresis(CZE),electrophoretic separation was achieved with 50 mmol/L phosphate 12.5 mmol/L borate (pH 8.08) running buffer and a constant voltage of 20 kV applied to the uncoated capillary(48 cm×50 μm).The analytes were introduced into capillary by pressure(12 psi×s) and determined with UV monitoring at 265 nm. An ideal separation for cefaclor and internal standard cefradine in human plasma was completed within 6.5 min by HPCE and no interference was found in their peak situations. The good linear relationship was obtained within the range of cefaclor plasma concentration 0.25~\{25.0\} μg/ml (\%r=0.9993,n\%=5). The absolute recovery was more than 90.5% and the intraday or interday precision in replicate injections(\%n\%=5) was less than 9.5%.This HPCE method has been applied successfully to dynamic analysis of clinical plasma samples after a single dose oral administration of 500 mg cefaclor capsule to six healthy volunteers