红景天中8种成分体外抗氧化作用的比较

Comparison on the antioxidant activity of eight components from Rhodiola in vitro

目的比较性研究红景天中8种苷及醇类化合物———芦丁苷、红景天苷、异槲皮苷、酪醇、络塞琳、熊果苷、络塞维、肉桂醇红景天的体外抗氧化作用。方法测定8种成分对DPPH自由基、超氧阴离子自由基、一氧化氮自由基、羟自由基的清除能力,以及总抗氧化能力和抑制脂质过氧化反应的能力。结果除清除羟自由基实验外,异槲皮苷和芦丁苷作用明显强于其他化合物,其中在清除DPPH、超氧阴离子能力和抑制脂质过氧化实验中的IC50分别为5.72和10.46,23.63和36.39,10.78和56.49 mg.L-1;而在清除羟自由基实验中,酪醇和红景天苷的作用则强于其他化合物,IC50分别为10.84和18.97 mg.L-1。结论红景天中苷及醇类成分对多种自由基具有清除作用,有良好的综合抗氧化性和开发应用前景。

Aim To make a comparative study of the antioxidant activity of eight kinds of glycosides or alcohols from Rhodiola in vitro, including Rutin, Salidroside, Isoquercitrin, Tyrosol, Rosarin, Brbutin, Rosavin, Cinnamyl alcohol. Methods The antioxidant activities were evaluated by total anti-oxidative capacity assay, antihpid peroxidation capacity assay, 1,1-di- phenyl-2-picrylhydrazyl （DPPH） free radical scavenging activity assay, superoxide anion scavenging activity assay, NO free radical scavenging activity assay and hydroxyl scavenging activity assay. Results Isoquercitrin and Rutin showed significantly stronger antioxidant activity than other components in all assays excepthydroxyl scavenging. In DPPH radical scavenging, superoxide anion radical scavenging, antilipid peroxidation assays, IC50 value were 5.72 and 10. 46, 23.63 and 36. 39, 10. 78 and 56. 49 mg· L^-1 respectively. While Tyrosol and Salidroside showed stronger antioxidant activity in hydroxyl scavenging, their IC50 were 10. 84 and 18.97 mg · L^-1. Conclusions Glycosides or alcohols from Rhodiola have scavenging activities on various of free radicals and have excellent antioxidation property and a prospective future of applications.