基因重组乳链球菌防龋疫苗免疫性的实验研究

Experimental Study on the Immunity of Recombinant Streptococcus Lactis Anticaries Vaccine

【目的】测定基因重组乳链球菌防龋疫苗的免疫原性和免疫反应性。【方法】用基因重组乳链球菌 (HL 10 7)、乳链球菌 (LM 0 2 30 )、变形链球菌Ingbritt分别灌胃免疫实验动物BALB/c鼠 ,与未免疫组作对照 ,以酶联免疫吸附实验 (ELISA)测定血清及唾液中的抗变形链球菌表面蛋白PAc的特异性IgG和IgA。【结果】基因重组乳链球菌组血清中抗PAc IgG水平明显高于乳链球菌组 (P <0 0 1)、变形链球菌组 (P <0 0 5 )和未免疫组 (P <0 0 1) ,唾液中重组乳链球菌组抗PAc IgA抗体水平明显高于乳链球菌组 (P <0 0 1)和未免疫组 (P <0 0 1) ,与变形链球菌组无差别 (P >0 0 5 ) ,血清IgA和唾液IgG的水平在 4组中均无差别 (P >0 0 5 )。【结论】基因重组乳链球菌具有变形链球菌表面蛋白的免疫原性 。

Objective To test the immunogenicity and immunoreactivity of recombinant S. lactis. Method The intragastric immunization was performed individually with gene recombinant S.lactis (HL 107), S.lactis (LM 0230) and S.mutans Ingbritt in BALB/c mice.Saliva and serum samples were assayed by enzyme linked immunosorbent assay (ELISA). Result The recombinant S. lactis were more effective in inducing PAc specific serum IgG antibodies in BALB/c mice than the other groups when given intragastrically(P<0 05). The level of PAc specific salivary IgA in the group of recombinant S. lactis was higher than the other groups (P<0 01) except S. mutans Ingbritt (P>0 05). PAc specific salivary IgG and PAc specific serum IgA in all groups showed no difference (P>0 05). Conclusion The gene recombinant S. lactis possesses an immunogenicity of S. mutans surface protein antigen and induces specific immune response in BALB/c mice.