口腔鳞癌转移淋巴结淋巴细胞的增殖及杀伤活性

Proliferative Ability and Cytotoxicity of Metastatic Lymph Node Lymphocytes from Oral Squamous Cell Carcinoma

:【目的】观察口腔鳞癌肿瘤浸润淋巴细胞和转移淋巴结淋巴细胞 ,哪一种更适于作过继免疫治疗。【方法】从 15例口腔鳞癌原发灶及其中 5例转移灶中分离肿瘤浸润淋巴细胞和转移淋巴结淋巴细胞 ,体外培养 ,观察两种来源的淋巴细胞增殖能力 ;用3H -TdR 4h释放法对 5例口腔鳞癌的肿瘤浸润淋巴细胞和转移淋巴结淋巴细胞对自体肿瘤杀伤活性进行比较。【结果】转移淋巴结淋巴细胞较肿瘤浸润淋巴细胞扩增高峰延迟 1周 ,转移淋巴结淋巴细胞培养 5周后仍显示强有力的扩增潜力。 3周时肿瘤浸润淋巴细胞对自体肿瘤的杀伤活性较转移淋巴结淋巴细胞强 (P <0 0 2 ) ,分别为 35 %和 2 3 % ;4周时转移淋巴结淋巴细胞对自体肿瘤的杀伤活性大于肿瘤浸润淋巴细胞 (P <0 0 2 ) ,分别为 5 3 %和 40 %。【结论】在口腔鳞癌患者的过继免疫治疗中 。

Objective To observe tumor infiltrating lymphocyte(TIL) and metastatic lymph node lymphocyte(MLNL) from patients with oral squamous cell carcinoma (OSCC), which has greater potentiality for adoptive immunotherapy. Methods TIL were isolated from fresh tumor tissues in 15 patients with OSCC and MLNL were obtained from metastatic lymph nodes in 5 patients with OSCC. The proliferative ability of TIL and MLNL were observed. The cytotoxicity against autologous tumor cells of TIL and MLNL were analyzed comparably using 3H TdR 4 h release method. Results The proliferative peak of MLNL was one week later than TIL, which exhibited strongly expansive potentiality after 4 weeks in culture. Culturing for 3 weeks, the cytotoxicity against autologous tumor cells of TIL was stronger than MLNL (35% vs 23%, P<0 02), while culturing for 4 weeks,the latter became stronger than the former (53% vs 40%, P<0 02). Conlusion These results suggest that using MLNL as adoptive immunotherapy for patients with OSCC has greater potentiality than using TIL.