PEEK/HA复合材料热稳定性及细胞毒性试验

PEEK/HA composite materials biocompatibility

目的制备PEEK/HA生物复合材料,研究该材料的热性能和生物相容性。方法在缩聚合成聚醚醚酮的基础上,与10%、20%、30%的纳米羟基磷灰石混合,制成聚醚醚酮/羟基磷灰石﹙PEEK/HA﹚生物复合材料。用热重分析法研究复合材料热稳定性。采用差示扫描量热仪﹙DSC﹚研究纳米羟基磷灰石对PEEK结晶动力学的影响。GFP、RFP荧光蛋白转化的大肠杆菌,与材料一起培养,观察材料对荧光蛋白表达影响。WST-1试剂盒检测该材料对Hela细胞毒性。结果 PEEK/HA复合材料热分解起始温度在500℃以上,热稳定性好。在加入20%纳米羟基磷灰石时,聚醚醚酮复合材料的结晶活化能﹙E﹚低,易于形成结晶。PEEK/HA复合材料对原核细胞和Hela细胞无明显毒性。结论 PEEK/HA生物复合材料的热性能和生物相容性良好。

Objective To prepare PEEK/HA composite materials, study the material thermal properties and blOcompatl- bility. Method Based on the polycondensation of polyether-ether-ketone, adding 10%, 20%, 30% hydroxyapatite blending into PEEK, prepared biological composite materials. Thermogravimetric analysis is to study thermal stability of the composite materials. By differential scanning calorimetry （ DSC ） the effect of HA on PEEK crystallization was studied. Results The thermal decomposition temperatures are above 500 ~C in starting, showing thermal stability is good. The Crystallization activation energy of the composite materials is lower than that of PEEK. Cytotoxicity test shows that PEEK/HA composites have no significant toxicity to prokaryotic cells and Hela cells. Conclusion Thermal properties and biological compatibility of PEEK/HA composites is good.