苦参不同制剂及成分对合轴马拉色菌标准株体外抑菌实验研究

Inhibitive effects study of different preparations and compositions of Matrine on standard strains of Malassezia sympodialis in vitro

目的观察苦参的不同制剂(粉剂水提、免煎剂)及苦参碱对合轴马拉色菌标准株的抑菌作用,探讨中药对合轴马拉色菌的作用及机制。方法将实验对象分为7组:苦参粉水提组、苦参免煎颗粒组、苦参碱组、氧化苦参碱组、酮康唑组,还设了不含药物的阳性组和不含菌的阴性组。通过不同的马拉色菌培养基对苦参的不同制剂及苦参中主要生物碱进行体外抑菌研究,并同酮康唑相对照,同时测定各自的最小抑菌浓度(MIC值)、最佳抑菌浓度。结果五组的MIC值从低到高分别是:苦参碱<氧化苦参碱<酮康唑<苦参颗粒<苦参粉水提组,苦参碱组的抑菌作用比较明显,MIC值为0.3mg/ml,低于其他各组的MIC值。结论苦参的主要生物碱中苦参碱具有较好的体外抑制马拉色菌作用,氧化苦参碱与酮康唑的体外抑菌作用相当,而苦参水提剂及苦参颗粒剂体外抑菌作用较差。推测苦参碱是苦参中主要起抑菌作用的有效成分。

Objective Comparing Malassezia Sympodialis fungistasis of water extract of KuShen powder, KuShen granules, matrine, oxymatfine and Ketoconazole to discuss the mechanism of Chinese medicine. Methods 7 groups （KuShen powder, KuShen granules, matrine, oxymatrine and Ketoconazole） were arranged to test the inhibitive effects of different preparations and compositions of Matrine on standard strains of Malassezia sympodialis in vitro, and compare the effects with positive control group without medicine, negtive control group without Malassezia Sympodialis, and Ketoconazole group. We observed growth of Malassezia Sympodialis and detected MIC（ the Minimum inhibitory concentration） and BIC（the Best inhibitory concentration） of the treatment groups. Results Every group had inhibitive effects on standard strains of Malassezia sympodialis in vitro. MIC of all the groups from low to high is matrine group, oxymatrine group, Ketoconazole group, KuShen granules group, water extract of KuShen powder group. Matrine had the best efficacy. Conclusion The main alkaloid matrine in KuShen has best inhibitory effect of Malassezia. Oxymatrine has similar function to ketoconazole. KuShen granules and Water extract of KuShen powder showed no obvious inhibitive effects. We may speculate that matrine is the main effective component of Kushen.