Akt/FoxM1在VB-1诱导的人宫颈癌HeLa细胞凋亡中的作用

Effects of Akt/FoxM1 Signaling Pathway on Apoptosis of Human Cervical Cancer HeLa Cells Induced by VB-1

目的研究Akt/FoxM1在新木脂素(VB-1)诱导的人宫颈癌HeLa细胞凋亡中的作用。方法采用不同浓度VB-1处理HeLa细胞后,通过MTT法测定细胞活力,琼脂培养集落形成法检测细胞集落形成能力,Histone/DNAELISA和琼脂糖凝胶电泳检测细胞凋亡,Western Blot检测p-AKT和FoxM1的表达水平。结果 VB-1以浓度依赖的方式显著抑制HeLa细胞的活力和集落形成,提高细胞内Histone/DNA片段化水平,呈现出典型DNA梯形条带,同时下调p-AKT和FoxM1表达水平。结论 VB-1能以浓度依赖性方式有效诱导人宫颈癌HeLa细胞凋亡,其作用机制可能与下调p-AKT和FoxM1有关。

Objective To investigate the role of Akt/FoxM1 signaling pathway in apoptosis process of human cervical cancer Hela cells induced by novel lignan VB-1. Methods Human cervical cancer HeLa cells were cultured in vitro. Cell viability was examined using MTT assay. Colony formation in HeLa cells was detected using clonogenic assay, and apoptosis was detected by Histone/DNA ELISA and agarose gel electrophoresis. Expression ofp-AKT and FoxM1 was examined by western blot analysis. Results VB-1 significantly inhibited the cell viability and colony formation, increased the histone/DNA fragmentation level, down-regulated the expression of p-AKT and FoxM1 in HeLa ceils in a concentration-dependent manner. The classic DNA ladder bands appeared in different concentrations of VB-1-treated HeLa ceils. Conclusions Neolignan VB-1 was capable of effectively inducing the apoptosis of human cervical cancer HeLa cells in a concentration-dependent manner. The down-regulation of AKT/FoxM1 pathway may be involved in the process.