高效液相色谱法同时测定解郁和肝胶囊中栀子苷和芍药苷的含量

Determination of geniposide and paeoniflorin in Jieyu Hegan capsules by HPLC

目的:建立解郁和肝胶囊中栀子苷和芍药苷的HPLC含量测定方法。方法:用Diamonsil-C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.1%磷酸溶液(14∶86)为流动相;检测波长为238 nm;柱温为25℃;流速为1.0 mL·min-1。结果:栀子苷进样量在0.16～2.42μg范围内,进样量与峰面积呈良好的线性关系,r=0.999 7,平均回收率为99.25%(n=6);芍药苷进样量在0.12～1.82μg范围内,进样量与峰面积呈良好的线性关系,r=0.999 5,平均回收率为98.87%(n=6)。结论:本方法操作简便,结果准确,重现性好,可作为该制剂的含量测定方法。

Objective：To establish a HPLC method for the determination of geniposide and paeoniflorin in Jieyu Hegan capsules. Methods：The HPLC analysis were carried out on a Diamonsil-Ci8 column （250 mm×4.6 mm, 5 μm） using acetonitrile-0.1% phosphoric acid （14： 86） as the mobile phase, the UV detection was carried out at 238 nm, the temperature was 25 ℃ , and the flow rate was 1.0 mL. min^-1. Results： Geniposide showed good line- arity in the range of 0.16- 2.42 μg （ r = 0. 999 7 ） , and the average recovery was 99.25% （ n = 6 ） ; paeoniflorin showed good linearity in the range of 0. 12 -1.82μg （r =0. 999 5） ,and the average recovery was 98.87% （n =6）. Conclusion：The method is simple,accurate,reproducible,and can be used for the determination of Jieyu He- gan capsule.