广东汉族人群HLA-B~* 15等位基因分布

HLA-B~* 15 diversity in Guangdong Han population

目的了解广东献血者汉族人群HLA-B*15等位基因的多态性。方法从1 691名广东汉族献血者中采用中/低分辨分型获得带有HLA-B*15基因型466例样本,进一步应用PCR-SBT技术进行测序分型,获得广东汉族人群B*15高分辨分型结果。应用PyPop软件计算HLA-B*15各等位基因频率,同时与其他人群资料进行比较。结果共检测到16种已知HLA-B*15等位基因,其中以B*15∶02(64.75%)最常见,其次为B*15∶01(13.26%),B*15∶25(4.554%),B*15∶27(4.158%),B*15∶12(3.960%),这5种等位基因占B*15等位基因家族的90.69%。在B*15等位基因家族中,表达B75抗原的等位基因B*15∶02、B*15∶11和B*15∶21共占67.92%,但表达B62抗原的B*15等位基因多态性最强,共检出6种等位基因。结论广东汉族人群中HLA-B*15等位基因表现出高度的多态性及其特有的分布特征。

Objective To investigate the allele distribution of HLA-B＊ 15 gene family in GuangDong Han population. Methods A total of 466 samples with HLA-B＊ 15 genotypes were selected from 1691 GuangDong Han individuals,based on their HLA-B types determined by PCR-SSP or PCR-SSO using commercial typing kits. PCR-SBT was applied to determine HLA-B＊ 15 allele typing. Allele frequencies were analyzed by PyPop Software. And the chi-square values of allele frequencies from different populations were also analyzed. Results A total of 16 different HLA-B＊ 15 alleles were identified. B＊ 15：02（64.75%） was the most frequent allele,followed by B＊ 15：01 （13.26%）,B＊15：25 （4.554%） ,B＊ 15：27 （4. 158%）,B＊15： 12（3. 960%）, which comprised more than 90. 69% of all the B＊15-positive samples. In the B62 serologic group,seven alleles were identified, including B＊15 ： 01, B＊1505 ,B ＊ 15：25 ,B ＊ 15：27 ,B ＊ 15：35 ,B＊ 1： 558. In the B75 serologic group,B＊15：02 and B＊15：11 were identified in the study samples and B ＊ 15：02 was predominant. Further research is required to identify this unknown allele. Conclusion Our study demonstrates that the high diversity of B＊ 15 in the GuangDong Han population,and provide that the basic data for the guidance of transfusion and drug hypersensitivity.