摘要
乌桕梓油中含有一种特殊结构的四酯成分。利用柱层析技术对乌桕梓油中的四酯进行分离。采用紫外分光光度法(UV)、气相色谱(GC)、气相色谱-质谱联用(GC-MS)和高效液相色谱(HPLC)对分离样品进行分析。结果表明,柱层析适宜的分离条件为:硅胶为吸附剂,95%石油醚和乙酸乙酯的混合溶液为洗脱剂,洗脱剂流速为1 mL/min,硅胶用量为17 g/g梓油。在此条件下可以得到纯度为98%的梓油四酯产品,其收率为54.8%。
Stillingia oil are extracted from the seeds of Sapoum sebifrrum Roxb. It contains a special structure of tetracylglycerols. Tetracylglycerols in stillingia oil is isolated using column chromatography. The UV spectrometry, Gas Chromatography (GC), Gas Chromatography- Mass Spectrometry (GC -MS) and high performance liquid chroma- tography (HPLC) are used to analyze the separated samples. The result shows that the column chromatography is preferably conducted under such conditions: Silica gel as adsorbent, 95% of petroleum ether and 5% ethyl acetate mixtures as eluent,fiow rate of lml/min, m( stillingia oil ) :m(silica) -- 1: 17. The purity of 98% of stillingia oil samples is obtained and the yield is 54.8% under this condition.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2012年第8期52-56,共5页
Journal of the Chinese Cereals and Oils Association
基金
浙江省重大科技专项(2006C11015)
关键词
四酯
梓油
柱层析
tetracyglycerols, stillingia oil, column chromatography