乌桕梓油中四酯的分离及鉴定

Separation and Identification of Tetracylglycerols from Stillingia Oil

乌桕梓油中含有一种特殊结构的四酯成分。利用柱层析技术对乌桕梓油中的四酯进行分离。采用紫外分光光度法(UV)、气相色谱(GC)、气相色谱-质谱联用(GC-MS)和高效液相色谱(HPLC)对分离样品进行分析。结果表明,柱层析适宜的分离条件为:硅胶为吸附剂,95%石油醚和乙酸乙酯的混合溶液为洗脱剂,洗脱剂流速为1 mL/min,硅胶用量为17 g/g梓油。在此条件下可以得到纯度为98%的梓油四酯产品,其收率为54.8%。

Stillingia oil are extracted from the seeds of Sapoum sebifrrum Roxb. It contains a special structure of tetracylglycerols. Tetracylglycerols in stillingia oil is isolated using column chromatography. The UV spectrometry, Gas Chromatography （GC）, Gas Chromatography- Mass Spectrometry （GC -MS） and high performance liquid chroma- tography （HPLC） are used to analyze the separated samples. The result shows that the column chromatography is preferably conducted under such conditions： Silica gel as adsorbent, 95% of petroleum ether and 5% ethyl acetate mixtures as eluent,fiow rate of lml/min, m（ stillingia oil ） ：m（silica） -- 1： 17. The purity of 98% of stillingia oil samples is obtained and the yield is 54.8% under this condition.