中医痰证与APOA5基因c.553G>T多态性的关系研究

Study on Relationship between ApolipoproteinA5 Gene c.553G>T Polymorphism and Phlegm Stagnation Syndrome

目的:探讨载脂蛋白A5(APOA5)c.553G>T基因多态性与中医痰证的关系。方法:运用聚合酶链反应-限制性片段长度多态性分析技术(PCR-RFLP)对湖南地区131名冠心病等疾病的痰证患者及74名健康人的APOA5 c.553G>T基因型进行检测,同时检测所有研究对象的血脂、血压及体重指数。结果:205例受检者中,APOA5c.553G>T位点GG型177例(86.34%),GT型28例(13.66%),TT型0例(0%),G等位基因频率93.17%,T等位基因频率6.83%。痰证组体重指数、血压、总胆固醇、甘油三酯、低密度脂蛋白高于健康对照组,差异均有统计学意义(P<0.01);高密度脂蛋白低于健康对照组(P<0.05),但不同基因型之间血脂水平差异无统计学意义(P>0.05)。病例-对照研究提示c.553GT型是中医痰证发生的危险因素,但多因素Logistic回归分析不支持c.553GT是上述病证发生的独立危险因素。结论:APOA5c.553G>T基因多态性不是中医痰证的独立危险因素。

Objectve： To explore the relationship between the polymorphisms of apolipoproteinA5 （AFt）AS） gene （c.553G〉 T） and phlegm stagnation syndrome（PSS）. Methods： Patients of PSS suffering from the disease such as coronary heart dis- ease（n=131）, and healthy controls（n=74） were grouped and compared in this study. Using polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） technique to analyze the APOA5 （c.553G〉T） genotypes. Blood fat, blood pressure （BP） and body mass index（BMI） in the patients and controls were also measured. Results： The frequencies of GG and GT genotypes for the APOA5c.553G〉T polymorphism were 86.34%（n=177） and 13.66%（n=28）. The frequency of G allele was 93.17% and the frequency of T allele was 6.83%. The BMI,BP, TC, TG, and LDL in the PSS group were significantly high- er than that of the healthy controls（P〈0.01）. The HDL in the PSS group was lower than the healthy controls（P〈0.05）.But there was no significant difference between the different genotypes in blood fa levels（P〉0.05）. Patients-controls research indicates that c.553GT genotype was a risk factor of the PSS, but the Multinomial-logistic analysis didn＇t support it. Conclusion： The APOA5 gene c.553G〉T polymorphism wasn＇t the independent risk factor of the PSS.