大鼠应激时垂体c-fos和催乳素基因表达关系的研究

The Relationship Between c-fos and Prolactin Gene Expression in the Rat Pituitary after Restraint Stress

目的 :探讨应激刺激下 ,垂体是否有 c- fos表达及其与催乳素 (PRL)基因表达的关系。方法 :雌性 Wistar大鼠给予禁锢应激 6 0 m in,用原位杂交方法检测应激开始后 15 ,30 ,6 0 ,12 0 ,180 min时 PRL m RNA水平。结果 :基础状态下垂体前叶 c- fos有弱表达 ,在禁锢应激开始后 15～ 30 m in迅速增加达高峰 ,尔后下降 ,在 12 0～ 180 m in出现第 2次表达高峰 ,PRL m RNA水平的改变与 c- fos平行。结论 :垂体 c- fos可能参与介导应激引起的 PRL 基因的激活 ,垂体即刻早期基因的表达有可能作为神经内分泌系统激活的标志。

Objective: Our purpose was to explore if there is c-fos expression in pituitary and the relationship between c-fos and prolactin (PRL) gene expressions after stress. Methods: Wistar female rats were exposed to restraint stress for 60 min and killed at 15, 30, 60, 120, 180 min after stress. In situ hybridization was performed to detect the levels of c-fos and PRL mRNA in the anterior pituitary. Results: There was a low basal level of c-fos expression in the anterior pituitary. A rapid increase was noted from 15 to 30 min after restraint stress, then the expression declined. A second peak appeared at 120 to 180 min after stress. The change of PRL mRNA was parallel with the c-fos expression. Conclusion: c-fos might mediate the activation of PRL gene expression after stress. Immediate-early genes expression in pituitary may be a marker of activation of neuroendocrine system.