肺癌患者外周血单个核细胞分泌IL-1、IL-4、IL-6水平的研究

The Levels of Interleukin-1,Interleukin-4, and Interleukin-6 Production in Patients with Lung Cancer

目的 :研究肺癌患者外周血单个核细胞 (PBMCs)分泌 IL- 1、IL- 4、IL- 6的水平 ,及根治手术对其影响。方法 :分别采用生物学方法及酶联免疫方法测定 40例肺癌手术前、14例肺癌根治手术前后、30例正常对照者及 2 2例肺部良性疾病者细胞因子的水平。结果 :肺癌组分泌 IL- 1、IL- 4、IL- 6 (分别为 0 .5 6 6± 0 .10 2 ,7.918± 5 .0 71pg/ ml,3.499± 2 .0 88)均低于正常对照组 (分别为 0 .6 40± 0 .12 3,19.2 97± 10 .6 85 pg/ ml,5 .372± 0 .113,均 P <0 .0 1) ,且相互间无相关性。根治手术后产生 IL- 1、IL- 4水平明显提高 (分别为 t=5 .0 6 7,P <0 .0 1;t=2 .2 44 ,P <0 .0 5 )。结论 :检测 PBMCs分泌 IL- 1、IL- 4水平对肺癌的诊断有较好的敏感性和特异性 ,联合检测意义更大 ,且对监测疗效 ,提示预后也有重要的临床意义。

Objective:Our purpose was to study the levels of interleukin-1(IL-1), interleukin-4(IL-4), and interleukin-6(IL-6) in patients with lung cancer before and after radical therapy. Methods:We investigated fourty patients with lung cancer. Fourteen of them underwent radical operation. Thirty healthy controls and 22 pulmonary benign disorder controls were enrolled for possible confounded factors. Levels of IL-1 was detected by biological assay, IL-4 and IL-6 were detected by ELISA.Results:The levels of IL-1,IL-4, and IL-6 were significantly lower in lung cancer groups(0.566±0.102,7.918±5.071 pg/ml, 3.499±2.088, respectively)thanthoseinhealthy controls(0.640±0.123,19.297±10.685 pg/ml, 5.372±0.113, respectively, P< 0.01 ). Therewerenocorrelationamongthese cytokines.Furthermore, the activitiesofIL-1and IL-4 inpostoperativegroupswereobviouslyincreasedthanthoseinpreoperativeones(t=5.067,P<0.01, t=2.244, P<0.05, respectively) Conclusion:The levels of IL-1, IL-4 were relatively sensitive and specific for diagnosis of lung cancer, and they were also helpful in evaluating the therapeutic effect and prognosis.