中药腹腔灌洗对急性出血坏死性胰腺炎大鼠肠道细菌移位的影响

Effect of Abdominal Lavage with Chinese Drugs on Bacteria Translocation in Acute Hemorrhagic Necrotizing Pancreatitis in Rats

目的 :通过腹腔灌洗对制备的大鼠急性出血坏死性胰腺炎 (AHNP)模型治疗 ,观察各种腹腔灌洗液对AHNP时细菌移位的治疗作用。方法 :Wistar大鼠 16 0只 ,随机分成 :对照组、AHNP组、抗生素间断灌洗组、抗生素持续灌洗组、中药间断灌洗组、中药持续灌洗组 ,进行腹腔灌洗 ,并行血液、腹腔渗液细菌培养 ,胰腺病理检查。结果 :AHNP组胰腺病理损害严重 ,血液、腹腔渗液细菌培养阳性率分别为 80 %和 90 %。中药腹腔灌洗组与AHNP组比较 ,上述病理变化明显减轻 ,血液、腹腔渗液细菌培养阳性率均下降至 4 0 %。结论 :AHNP时肠粘膜屏障功能受损 ,发生细菌移位 ,导致肠源性胰腺感染。中药腹腔灌洗对防止细菌移位有一定的作用 ,也可减轻AHNP的病理损害。

To investigate the effect of different abdominal lavage fluid on bacterial translocation in acute hemorrhagic necrotizing pancreatitis (AHNP) of rat model. Methods: One hundred and sixty Wistar rats were randomly divided into the control group, AHNP group, antibiotics group and Chinese drugs group. The treated group were treated with intraperitoneal lavage for 72 hours. The peritoneal fluid and blood samples were collected for bacterial culture. Pancreas were examined histopathologically. Results: In Chinese drugs group, the pathologic damage in pancreas were milder than that of AHNP group, and as compared with AHNP group, the bacterial positive rates of blood and peritoneal lavage fluid culture were reduced from 80% to 40% and 90% to 40% respectively. Conclusion: AHNP impaired the gut barrier function which led to bacterial translocation from the gut to other organs. It plays an important role in intestinal infection secondary to AHNP. Abdominal lavage with Chinese drugs is effective in preventing intestinal bacterial translocation, it showed its protection on gut barrier function by alleviating the damage of intestinal mucosa and pancreatitis.