中药双龙丸对血管平滑肌细胞血小板衍化生长因子-A及单核细胞趋化蛋白-1mRNA表达的影响

Effects of Shuanglong Pill on mRNA Expression of Platelet-Derived Growth Factor A and Monocyte Chemoattractant Protein-1 mRNA

目的 :探讨中药双龙丸干预动脉粥样硬化的分子学作用机制 ,主要是对血小板衍化生长因子 A(PDGF A)、单核细胞趋化蛋白 1(MCP 1)mRNA表达的影响。方法 :采用培养的家兔动脉粥样硬化细胞模型 ,用高脂血清造成动脉粥样硬化细胞模型 ,用中药血清药理学方法将药物血清作用于细胞模型 ,采用逆转录 -聚合酶链式反应 (RT PCR)方法 ,观察双龙丸对PDGF A、MCP 1mRNA表达水平的影响。结果 :高脂血清能刺激细胞表达PDGF A、MCP 1mRNA ,双龙丸药物血清能使PDGF A、MCP 1mRNA表达水平降低。结论 :双龙丸能抑制细胞自分泌生长因子功能 ,这是其干预动脉粥样硬化的药理作用机制之一。

To explore the molecular mechanism of Shuanglong Pill (SLP) in intervening atherosclerosis, mainly the effect of SLP on the mRNA expression of the platelet derived growth factor A (PDGF A) and the monocyte chemoattractant protein 1 (MCP 1). Methods: Using the hyperlipoprotein serum (HLS) of cultured rabbits′ aortic smooth muscle cell (SMC )to build the atherosclerotic cell model, and medicated serum acting on the cell model by serum pharmacologic method. The effect of SLP medicated serum on the level of PDGF A, MCP 1 mRNA expression were observed and analysed by means of reverse transcriptase polymerase chain reaction assay. Results: It was found that HLS stimulated mRNA expression of the PDGF A and the MCP 1, but the medicated serum of SLP lowered their expression level. Conclusion: SLP could inhibit the function of cellular auto secreting growth factor, it is one of the possible pharmacologic mechanism in intervening atherosclerosis.