新生儿败血症病原菌及其药物敏感试验分析

Analysis of neonatal sepsis pathogens and their drug sensitivity test

目的了解杭州地区新生儿败血症病原菌的分布及药物敏感性,以指导临床合理应用抗生素。方法回顾性分析2009年1月～2011年5月我院新生儿病房临床诊断新生儿败血症97例,共分离出病原菌97株。结果 97株细菌中其中G+菌86株(88.7%),G-菌9株(9.2%),真菌2株(2.1%)。主要致病菌为表皮葡萄球菌49例(50.5%),其次是溶血葡萄球菌7例(7.2%);G-菌中大肠埃希菌占首位。G+菌对庆大霉素、莫西沙星、利福平、万古霉素敏感度较好;G-菌中大肠埃希菌对哌拉西林/舒巴坦、头孢西丁、万古霉素、亚胺培南/西司他丁敏感度较好。结论新生儿败血症主要病原菌为革兰阳性菌,葡萄球菌尤其是凝固酶阴性葡萄球菌是其重要致病菌。

Objective To understand the distribution and drug sensitivity of neonatal sepsis pathogens in Hangzhou area in order to guide the rational clinical application of antibiotics. Methods A total of 97 patients diagnosed with neonatal sepsis in the neonatal ward of our hospital from January 2009 to May 2011 were analyzed retrospectively. A total of 97 strains of pathogens were separated. Results Of the 97 strains of pathogens, 86 strains （88.7%） were G＋ bacteria, 9 strains （9.2%） were G- bacteria and 2 strains （2.1%） were fungi. The main pathogen was staphylococcus epidermidis, found in 49 patients （50.5%）, and followed by staphylococcus haemolyticus, found in 7 patients （7.2%）. Escherichia coli accounted for the ma- jority of G- bacteria. G＋ bacteria were sensitive to Gentamicin, Moxifloxacin, Rifampicin and Vancomycin; Escherichia coli of G- bacteria were sensitive to Piperacillin/Sulbactam, Cefoxitin, Vancomycin and Imipenem/Cilastatin. Conclusion The main pathogens of neonatal sepsis are Gram-positive bacteria, of which staphylococcus, especially coagulase-negative staphylococci is the important pathogen.