拟除虫菊酯对大鼠脑代谢型谷氨酸受体结合的影响

Effect of pyrethroids on the binding level of metabotropic glutamate receptor in rat brain

目的 比较两型拟除虫菊酯 (pyrethroid ,PY)类农药对大鼠脑代谢型谷氨酸受体(mGluR)结合功能的影响。方法 应用放射性配基受体结合试验在体外测定溴氰菊酯 (DM )和氯菊酯 (PM )对大鼠大脑皮层和海马突触膜mGluR结合功能的影响。结果 DM分别在 2× 10 -6、2× 10 -5、2× 10 -4 mol/L和 2× 10 -10 、2× 10 -8、2× 10 -6、2× 10 -4 mol/L剂量范围内直接增加大鼠大脑皮层和海马突触膜氚标记的谷氨酸 (3H Glu)与mGluR的特异结合量 ,分别为 :(10 7.6± 7.7)、(112 .4± 9.6 )、(115 .4± 12 .2 )fmol/mgpro .和 (15 9.8± 16 .9)、(16 6 .9± 2 0 .9)、(183.8± 2 1.2 )、(193.8± 2 5 .8)fmol/mgpro .;PM在 2× 10 -8～ 2× 10 -4 mol/L剂量范围内对大脑皮层突触膜3H Glu与mGluR的特异结合量无明显影响 ,在 2× 10 -6、2× 10 -4 mol/L时直接增加海马突触膜3H Glu与mGluR的特异结合量 ,分别为 :(173.8± 2 0 .1)、(180 .9± 2 4.3 )fmol/mgpro .。 结论 DM和PM均可不同程度地影响海马突触膜mGluR的结合功能 。

Objective To detect and compare the effect of two kinds of pyrethroid insectcides on the binding function of metabotropic glutamate receptor(mGluR) in rat brain.Methods Radio labelled ligand receptor binding assay was used to determine the effect of deltamethrin(DM) and permethrin(PM) on the binding function of mGluR in postsynaptic membrane of cerebral cortex and hippocampus.Results The binding levels of 3H glutamate to mGluR in postsynaptic membrane of cerebral cortex and hippocampus by DM were (107.6±7.7),(112.4±9.6),(115.4±12.2) fmol/mg pro. and (159.8±16.9),(166.9±20.9),(183.8±21.2), (193.8± 25.8) fmol/mg pro. at the dose of 2×10 -6 ,2×10 -5 ,2×10 -4 mol/L and 2×10 -10 ,2×10 -8 , 2×10 -6 , 2×10 -4 mol/L respectively.No obvious change in the binding levels of mGluR in postsynaptic membrane of cerebral cortex was observed by PM within the dose of 2×10 -8 ～ 2×10 -4 mol/L.The binding levels of mGluR in postsynaptic membrance of hippocampus by PM within the dose of 2×10 -6 , 2×10 -4 mol/L were (173.8±20.1),(180.9±24.3) fmol/mg pro. respectively. Conclusion Both pyrethroids could affect the binding function of mGluR in postsynaptic membrane of hippocampus by different degree.The binding function of mGluR could be more easily enhanced by DM with CN radical than PM without CN radical.