双波长薄层扫描法测定消炎利胆片中穿心莲内酯的含量

Determination of Andrographolide in Xiaoyan Lidan Pian by TLC Scanning Method

目的 :测定消炎利胆片中穿心莲内酯的含量。方法 :双波长薄层扫描法 ,波长λs=2 2 5nm ,λR=2 75nm。结果 :穿心莲内酯含量线性范畴 2 .0 2～ 10 .10 μg ,平均加样回收率为 98.86 % ,RSD为 0 .5 0 %。结论 :本测定方法简便、准确、重现性好 。

Aim To detemine andrographolide in Xiaoyan Lidan pian.Methods The TLC method was used.The value λ S of λ R were 225 and 275nm respectively.Results The linear range of the method was 2.02～10.10μg.The correlation coefficient was 0.999 1.The recovery could reach 98.86% and the relative standard deviation RSD was 0.50%.Conclusion The method was proved to be simple,accurate and reproducible.