不同年份林下山参叶中皂苷类成分含量变化研究

Research on ginsenosides composition in wild ginseng leaves in different growing years

目的:分析不同年份林下山参叶中多个人参皂苷类成分的含量,考察年份对人参皂苷类成分含量的影响。方法:采用Acquity UPLC BEH Shield RP18(2.1 mm×100 mm,1.7μm)色谱柱,流动相为乙腈-0.05%甲酸溶液梯度洗脱;检测波长203 nm。结果:建立了同时测定13个人参皂苷类成分的含量的UPLC分析方法,分析了5～17年份林下山参叶中人参皂苷类成分的含量,皂苷含量随年份有波动,14年份各皂苷类成分含量均处于较高水平。结论:建立的分析方法,简便,准确,可靠,可以作为同时测定人参类药材中多种皂苷含量的方法及指纹图谱研究方法;研究结果为林下山参叶人参皂苷类成分的积累和林下参资源的利用提供了可靠的基础。

Objective： To analyze ginsenosides composition in wild ginseng leaves with different growth years. Method： The analysis was performed on Acquity UPLC BEH Shield RP18 （2.1 mm×100 mm, 1.7 μm） column, the mobile phase was acetonitrile-0.05% formic acid solution in gradient elution mode.The detection wavelength was at 203 nm.The flow rate was 0.4 mL·min-1 and column temperature was set at 30℃. Result： Thirteen ginsenosides were determined by the established UPLC method.In 5-17th growth year ginseng leaf samples cultivated simulating wild conditions, the contents of ginsenosides in the 14th year have the highest content. Conclusion： The established method is simple, accurate and reliable, can be used in ginsenosides determination and fingerprint research of Panax crude drug.The result provides reliable data for the accumulation of ginsenosides and sustainable utilization of ginseng resources.