滚筒杀青烘干加工方法对四倍体金银花药材质量的影响

Effect of gas-turbine green discoloring and drying processingmethods on herbal quality of tetraploid Lonicerae Japonicae Flos

目的:研究滚筒杀青烘干加工方法对不同品种金银花药材质量的影响。方法:应用高效液相色谱仪Waters1525,采用DIKMA DiamonsilTM-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸溶液,梯度洗脱;流速1.0 mL.min-1;柱温25℃;检测波长355 nm。结果:滚筒杀青烘干加工的四倍体金银花呈绿色,其绿原酸和木犀草苷质量分数分别为5.31%,0.105%,两者分别显著高出相同加工方法下二倍体的18.0%,32.1%,并且滚筒杀青烘干的四倍体金银花绿原酸含量显著高于四倍体和二倍体金银花的传统自然晒干处理。结论:滚筒杀青烘干方法是适合四倍体金银花的新型干燥方法;在滚筒杀青烘干加工条件下,四倍体金银花药材质量显著优于原亲本二倍体,是值得推广应用的优良品种。

Objective： To study the effect of gas-turbine green discoloring and drying processing method on the quality of various Lonicerae Japonicae Flos herbs. Method： DIKMA DiamonsilTM-C18 column （4.6 mm×250 mm, 5 μm） was adopted using HPLC Waters 1525 and eluted with acetonitrile and 0.1% phosphate acid as the mobile phase. The flow rate was 1.0 mL·min-1, the column temperature was 25℃ the detection wavelength was 355 nm. Result： After being processed by the gas-turbine green discoloring and drying method, tetraploid Lonicerae Japonicae Flos showed a green color. The contents of chlorogenic acid and galuteolin were 5.31% and 0.105%, both significantly higher by 18.0% and 32.1% than those of diploid Lonicerae Japonicae Flos processed by the same method. The content of chlorogenic acid in tetraploid Lonicerae Japonicae Flos processed the gas-turbine green discoloring and drying method were also remarkably higher than that of tetraploid and diploid Lonicerae Japonicae Flos processed by traditional processing method of natural drying. Conclusion： The gas-turbine green discoloring and drying processing method is a new-type drying method suitable for tetraploid Lonicerae Japonicae Flos. Under the condition of gas-turbine green discoloring and drying processing, tetraploid Lonicerae Japonicae Flos shows much higher quality than Lonicerae Japonicae Flos, suggesting that it is a good variety worth popularizing and applying.