RP-HPLC测定大黄中4个苯丁酮类化合物

Determination of four n-butyrophenone compounds contained in rhubarb using RP-HPLC

目的:建立大黄中4-4'-羟基苯基-2-丁酮,莲花长苷,异莲花长苷及4'-羟基苯基-2-丁酮-4'-O-β-D-(2″-O-桂皮酰基-6″-O-没食子酰基)-葡萄糖苷的含量测定方法。方法:Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.05%磷酸溶液梯度洗脱,流速1 mL.min-1,检测波长268 nm。结果:4个成分的分离度良好,标准曲线在检测范围内均呈良好线性(r>0.999 9),其检测限均低于1.76 ng,定量限均低于4.98 ng,高、中、低3个水平日内和日间精密度的RSD均小于2.3%,加样回收率分别均高于91.8%。结论:该方法简便、快速、灵敏、准确,可为大黄的全面质量评价提供参考。

Objective： To establish the method for determining the contets of 4-（4＇-hydroxyphenyl）-2-butanone, lindleyin, isolindleyin, and 4-（4＇-hydroxyphenyl）-2-butanone-4＇-O-β-D-（2″-O-galloyl-6″-O-cinnamoyl）-glucopyranoside contained in rhubarb. Method： Agilent Zorbax SB-C18 analytical column （4.6 mm×250 mm, 5 μm） was adopted as the chromatographic column, with acetonitrile-0.05% phosphoric acid as mobile phase for gradient elute. The flow rate was 1 mL·min-1, and detective wavelength was set at 268 nm. Result： 4-（4＇-hydroxyphenyl）-2-butanone, lindleyin, isolindleyin, and 4-（4＇-hydroxyphenyl）-2-butanone-4＇-O-β-D-（2″-O-galloyl-6″-O-cinnamoyl）-glucopyranoside showed good resolutions. The four standard curves displayed good linear relationship within the detection rate （r〉0.999 9）, with their detection limit of less than 1.76 ng and quantitation limit of less than 4.98 ng. At the high, medium and low levels, their RSDs of inter-and intra-day precision were less than 2.3%, with recovery of more than 91.8%. Conclusion： The method is so simple, rapid, accurate and reliable that it can provide reference for comprehensive quality evaluation on rhubarb.