pH值对SBBR自养脱氮系统效能及功能菌数量的影响

Effect of pH on SBBR Autotrophic Nitrogen Removal Process

从在不同pH值条件下稳定运行的SBBR自养脱氮反应器中分别提取活性污泥及生物膜样品的基因组DNA,通过特异引物扩增系统内亚硝化菌(AOB)、硝化菌(NOB)及厌氧氨氧化(ANAMMOX)菌的基因序列,采用荧光定量PCR技术对功能菌进行定量分析,研究pH值对系统运行效能及功能菌数量的影响。研究显示,pH值对SBBR自养脱氮系统的运行效能及三大功能菌的数量均有显著影响。在pH值=6.0的酸性条件下,AOB、NOB及ANAMMOX菌数量比其他pH值条件下的低,脱氮效果差。AOB在pH值=7.0～8.0范围内数量较多,在pH值=9.0时将受到明显抑制。在pH值=7.0～9.0的范围内,NOB数量的变化趋势没有AOB的显著。在pH值=8.0时,各功能菌的数量均达到最大值,系统构成一个和谐稳定的微生态环境,运行效能较佳,因此该值为此SBBR自养脱氮系统的最佳控制点。

The effects of pH on ammonia oxidizing bacteria （AOB）, nitrite oxidizing bacteria （NOB）and anaerobic ammonium oxidizing （ANAMMOX） bacteria and on the overall nitrogen removal performance of SBBR were investigated. Specific PCR primers were used to determine the population of functional bacteria in the SBBR process. The results indicated that the nitrogen removal performance and ＇it, the populations of AOB, NOB and ANAMMOX bacteria were greatly influenced by pH. The populations of all three types of bacteria were the lowest at pH 6. O. AOB thrived in the ＂pH range of 7.0 to 8. O, but was inhibited at pH 9.0. No significant change in NOB population was observed in the pH range of 7.0 to 9. O. The populations of all the bacteria were the highest at pH 8.0. The SBBR achieved maximum ni- trogen removal performance when operated at pH 8.0.