大鼠血浆中麻黄碱伪麻黄碱含量的高效液相色谱法测定

Determination of Ephedrine and Pseudoephedrine in Plasma by HPLC

目的建立高效液相色谱(HPLC)法测定大鼠口服中药复方麻黄制剂后血浆中麻黄碱、伪麻黄碱的含量,为研究中药复方麻黄制剂在大鼠体内药动学提供基础。方法采用Synergi 4u Polar-RP 80A(250 mm×4.60 mm,4μm)色谱柱,以甲醇-0.092%磷酸(含0.04%三乙胺、0.02%二正丁胺)为流动相,流速为1.0 ml/min,柱温为25℃,检测波长为207nm。结果麻黄碱在1.43～45.76μg/ml范围内线性关系良好(r=0.999 8),伪麻黄碱在1.35～43.12μg/ml范围内线性关系良好(r=0.999 8),回收率符合生物样品分析,两者日内、日间精密度的RSD<5%。结论建立的高效液相色谱测定方法为动物体内麻黄碱、伪麻黄碱的测定提供了一个可靠的方法。

Objective To establish the method for determination of ephedrine and pseudo - ephedrine in rat plasma with high performance liquid chromatography. Methods Ephedrine and pseudo - ephedrine were separated on Synergi 4u Polar - RP 80A column （250 mm × 4.60 mm,4 um） , with mobile phase consisted of methanol - 0.092 % phosphoric acid （ containing 0.04% triethanolamine and 0.02% Di- n -Butyl Amine） at a flow rate of 1.0ml/min, the column temperature was 25℃ ,and the detection wavelength was set at 207 nm. Results The calibration curves of E and PE were linear within the range from 1.43 ug/ml to 45.76 ug/ml （ r = 0. 9998 ） and from 1.35 ug/ml to 43.12 ug/ml （ r = 0.999 8 ）, respectively. The RSDs of interday and intraday were all less than 5%. Conclusion The method can be used to determine ephedrine and pseudo - ephedrine in vivo.