五种凝集素受体在膀胱癌中的分布及意义

Distribution of five lectins receptors in bladder carcinoma and their significance

目的 探讨膀胱癌中凝集素受体的分布与其分化程度、浸润深度、转移和预后的关系。方法 应用生物素标记的花生凝集素 (PNA)、麦胚凝集素 (WGA)、扁豆凝集素 (LCA)、刀豆凝集素 (ConA)及菜豆凝集素 (PHA)等 5种凝集素对 5 2例人体膀胱癌 ,10例正常人体膀胱粘膜进行亲合组织化学法研究。结果 发现正常膀胱粘膜PNA、WGA及PHA受体阴性 ,PNA受体阳性率随膀胱癌病理分级的上升而递增 ,相互之间差异具有显著性 (P <0 0 5 )。PNA、WGA及PHA受体阳性率在浸润性肿瘤中明显高于浅表性肿瘤 (P <0 0 5 )。 84 0 %淋巴结转移癌与其原发癌的凝集素标记的量上有差异 ,并且与原发肿瘤获得更多的PNA及PHA受体。术后 2a以上复发的膀胱癌中 ,PNA受体的阳性率小于 2a以内复发的膀胱癌中该受体的阳性率 (P <0 0 5 )。结论 PNA、WGA及PHA受体阳性率与膀胱癌浸润深度有关。PNA及PHA与淋巴结转移癌有关。PNA与膀胱癌分化程度有关 ,肿瘤组织内的PNA受体可作为膀胱癌术后预测预后的参考指标之一。

Objective To study whether the distribution of lectin receptors in the bladder carcinoma are relative to the degree of differentiation, invasion, metastasis and prognosis of the bladder carcinoma. Methods five lectins (Biotin PNA, Biotin WGA, Biotin PHA, Biotin ConA, Biotin LCA) were used to study 52 cases of human bladder carcinoma and 10cases of normal bladder mucosa, by using the affinity histochemical method. Results No PNA, WGA and PHA were found in normal bladder mucosa. The positive rate of PNA receptors showed progressively increase in the cases of bumor grade. The statistical analyses showed a significant difference between the above three groups (P<0.05). The positive rates of PNA, WGA and PHA receptors in invasive tumors were significant higher than that in the superficial ones (P<0.05). 84% lymph node metastatic tumors produced cellular glycoconjugates, which differed in lectin binding profiles from those made by the cells in the respective primary carcinoma. More PNA and PHA receptors were produced by the lymph node metastatic tumors than primary carcinoma. The tumors that recurred longer than two years after curative cystectomy had PNA receptors obvious fewer than those recurring within two years (P<0.05). Conclusions The positive rates PNA, WGA and PHA receptors are relative to the degree of invasion of bladder carcinoma. The positive rates of PNA and PHA receptors are relative to the lymph node metastatic tumors. The positive rates PNA receptors are relative to the degrees of differentiation of bladder carcinoma; It is suggested that PNA receptors in tumor tissues might be a useful index in estimating the prognosis of patients with bladder carcinoma after cystectomy.