四川产九牛造中总鞣质和没食子酸的含量测定

DETERMINATION OF TOTAL TANNINS AND GALLIC ACID IN THE CHINESE FOLK MEDICINE JIUNIUZAO GROWING IN SICHUAN

目的 : 测定九牛造三个品种中总鞣质和没食子酸的含量。方法 :用皮粉法测定总鞣质 ,薄层扫描法测定没食子酸。结果 :总鞣质和没食子酸含量分别为支柱蓼 7.386 %、0 .0 74% ;细穗支柱蓼 6 .2 65 %、0 .0 53 % ;抱茎蓼8.543 %、0 .0 61 %。没食子酸在 2 .2～ 1 1 .0 μg范围内线性关系良好 ,平均回收率 1 0 2 .42 % (n =6)。 结论 :方法简便、快速。

OBJECTIVE:To determine total tannins and gallic acid in Jiuniuzao's three species. METHODS:Tannins and gallic acid were determined by hide powder method and TLC scanning,respectively. RESULTS:The contents of tannins and gallic acid in Polygonum suffultum Maxim were 7.386% ,0.07367%; in P.suffultum Maxim.var. Pergracile (Hemsl.)Sam were 6.265%,0.053% and in P.amplexicaule D.Don were 8.543%,0.06116% respectively.The linearity of gallic acid was good within 2.2 11.0 μg. The average recovery in the sample was 102.42%( n =6). CONCLUSION:The methods were simple,rapid and accurate.