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活化T细胞核因子和单核细胞趋化蛋白-1及该蛋白2518G/A基因多态性与冠心病的关系 被引量：5

Relationship Between NFAT,MCP-1 With MCP-1-2518G/A Gene Polymorphism and Coronary Artery Disease
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摘要 目的:研究活化T细胞核因子和单核细胞趋化蛋白-1及该蛋白2518G/A基因多态性与冠心病病变程度的相关性。方法:选取冠心病患者300例,根据冠状动脉(冠脉)造影结果确诊为冠心病的患者分为急性冠脉综合征(ACS)组180例,稳定型心绞痛组120例。另选冠脉造影正常者为对照组60例。应用Gensini评分评定狭窄程度,酶联免疫法测定活化T细胞核因子(NFAT)、单核细胞趋化蛋白-1(MCP-1)浓度,多聚酶链反应--末端限制性片段长度多样性(PCR-RFLP)方法检测MCP-1 2518G/A基因多态性。结果:①急性冠脉综合征组的NFAT、MCP-1浓度显著高于稳定型心绞痛组及对照组,差异有统计学意义(P<0.05),MCP-1浓度与NFAT呈正相关(r=0.65,P<0.05)。②冠脉病变Gensini评分与NFAT、MCP-1、低密度脂蛋白胆固醇、血糖呈正相关,与高密度脂蛋白胆固醇呈负相关。③MCP-1 2518G/A基因有三个基因型,GG型的MCP-1浓度较AG型及AA型升高(P<0.05),ACS组GG型基因和G等位基因频率较稳定型心绞痛组及对照组增高,差异均有统计学意义(P<0.05)。结论:冠心病患者血浆NFAT与MCP-1水平的升高及MCP-1 2518G/A基因多态性与冠脉病变程度相关。 Objective ： To investigate the relationship between nuclear factor of activated T cells（NFAT） , monocyte chemoattractant pro- tein-1 （MCP-1） with MCP-1-2518G/A gene polymorphism and the severity of coronary artery disease （CAD）. Methods ：According to coronary angiography （CAG） examination, the patients were divided as Acute coronary syndrome （ACS） group, n = 180, Stable angina pectoris （SAP） group, n = 120 and Control group, n=60, the patients had normal CAG. The severity of coronary lesions was analyzed by Gensini coronary score system,the protein concentrations of NFAT and MCP-1 were measured by ELISA,and MCP-1-2518 G/A gene polymorphism was examined with PCR-RFLP. Results ：①The protein concentrations of NFAT and MCP-1 were both higher in ACS group than those in SAP group and Con- trol group,P〈0. 05 respectively. Plasma level of MCP-1 was positively related to NFAT （r=0. 65 ,P〈0. 05）. ②Gensini score of coronary lesions was positively related to NFAT, MCP-1, LDL-cholesterol and blood sugar, while negatively related to HI）L- cholesterol. ③There were 3 polymorphisms in MCP-1-2518G/A gene, GG type had higher MCP-1 than AG type and AA type, P〈0. 05 respectively. In ACS group, GG type and G Allen frequency were both higher than those in SAP group and Control group,P〈0. 05 respectively. Conclusion：The elevated plasma levels of NFAT, MCP-1 and MCP-1 gene polymorphism are related to the severity of coro- nary lesions in CAD patients.
作者 杨丽霞 郭瑞威 齐峰 石燕昆 王先梅 任丽 徐安妨
机构地区 成都军区昆明总医院心血管内科全军心血管病专科中心
出处 《中国循环杂志》 CSCD 北大核心 2012年第4期278-281,共4页 Chinese Circulation Journal
关键词 活化T细胞核因子 基因多态性 单核细胞趋化蛋白-1 冠心病 Nuclear factor of activated T cells Gene polymorphism Monocyte chemoattractant protein-I Coronary artery disease
分类号 R541.4 [医药卫生—心血管疾病]
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参考文献12

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共引文献2

同被引文献32

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中国循环杂志
2012年 第4期

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