转TaEBP基因小麦的回交转育研究

Tansfer of TaEBP Gene Into Cultivated Wheat Variety by Backcrossing

本研究以转TaEBP基因的新春9号小麦T 4代株系为供体,通过回交育种的方法将与抗逆相关的TaEBP转录因子基因导入黄淮麦区的7个主栽或主推小麦品种中,利用温室快速加代和目的基因PCR分子跟踪检测技术,实现了一年四代的快速繁育,在一年内获得了转基因回交三代株系群。PCR分析结果显示,TaEBP基因在杂交F1代植株中阳性率平均在72.3%以上,在6个轮回亲本的回交后代中基本符合1∶1分离比率,在刑麦六号的BC3F2代中呈3∶1分离,外源TaEBP基因的分布符合一对显性等位基因扩增结果的遗传分离比例;PEG-6000胁迫条件下,对3个来自刑麦6号BC3F2代的回交导入系苗期抗性鉴定显示,3个转基因回交导入系的抗旱性明显提高。表明利用转基因材料通过回交转育、快速加代和分子跟踪检测是快速定向改良小麦品种的性状、获得新的转基因小麦的一条有效途径。

Back-cross breeding combined with rapid generation-adding and target gene PCR molecular tracking detection was taken to transfer TaEBP genes to 7 wheat cultivars which are now widely used in Huang-huai wheat producing area. The transgenic BC3 Fl strain groups were obtained within one year. PCR analysis showed that the average positive rate of TaEBP gene in F1 hybrid was more than 72.3%, in the different backcross generation of 6 recurrent parents, the segregation ratio of genetic distribution of PCR-positive individual plants and PCR-negative indiVidual plants was about 1： 1, the ratio was 3：1 in BC3 F2 generation of Xingmai 6. The results showed that the distribution of exogenous TaEBP gene with a dominant allele amplification results of genetic ratio. Under PEG-6000 stress, the 3 interogression lines from Xingmai 6 BC3 F2 generations of baekcross introgression lines seedling resistance identification showed, the 3 transgenic introgression lines drought resistance increased obviously. The results confirmed that to transfer TaEBP gene into other wheat cuhivars by back-crossing,rapid generation-adding and target gene PCR molecular tracking detection is a simple and effective approach to getting a new transgenic wheat and directional improving resistance of wheat varieties.