单个核细胞趋化蛋白-1及其受体CCR2在多发性骨髓瘤基质细胞与瘤细胞中的表达

Expression of MCP-1 and its receptor CCR2 in tumor cells and stromal cells of multiple myeloma

目的观察单个核细胞趋化蛋白-1MCP-1及其受体CCR2在多发性骨髓瘤（MM）患者骨髓瘤细胞和骨髓基质细胞中的表达。方法经国内统-标准确诊为MM的患者15例，男7例，女8例，年龄38～67岁，平均年龄53．7岁。按照Durie—Salmon分期系统分期，I期2例、Ⅱ期5例、Ⅲ期8例。其他血液病患者10例为对照组。取样本进行骨髓瘤细胞与骨髓基质细胞的分离培养，在含13％胎牛血清的RPMI1640培养液中培养，每2d更换1次培养液，实验用细胞均处于对数生长期。采用流式细胞术检测骨髓瘤患者骨髓瘤细胞，骨髓基质细胞中MCP-1、CCR2水平。结果14例MM患者骨髓均表达MCP-1、CCR2，而对照组8例骨髓中趋化因子MCP-1、CCR2基本无表达。对照组和MM患者基质细胞上MCP-1、CCR2均阳性的表达率分别为4．27％和68．71％，两组比较差异具有统计学意义（P〈0．05）。瘤细胞上MCP-1、CCR2均阳性的表达率分别为3．25％和32．76％，两组比较差异具有统计学意义（P〈0．05）。处于疾病活动期的基质细胞和瘤细胞上MCP-1、CCR2均阳性的表达率分别为68．71％和32．76％，而处于疾病非活动期的的基质细胞和瘤细胞上MCP-1、CCR2均阳性的表达率分别为70．12％和53．39％，两组比较差异无统计学意义（P〉0．05）。结论大部分MM患者骨髓均表达MCP-1、CCR2。MCP-1及CCR2是MM细胞表面表达的主要趋化因子，对MM的疾病进展起着-个比较重要的作用。

Objective To observe the expression of chemokine （MCP-1） and chemokine receptor （CCR2） in bone marrow cells, bone marrow stromal cells of muhiple myeloma （MM） patients. Methods 15 cases were diagnosed by domestic uniform standard for MM patients, 7 cases of male, 8 cases of female, age range from 38 to 67 years, mean age 53.7 years old. According to the Durie-Salmon staging system, patients were divided into Ⅰ （2 cases）, Ⅱ （5 cases） and m period（8 cases）. Control group were from 10 cases of non-malignant blood disease patients. MCP-1, CCR2 expression were measured by flow cytometry. Results Almost 14 cases of bone marrow cells expressed MCP-1 and CCR2 in MM patients, while in the control group, bone marrow cells ahnost did not express MCP-1 and CCR2. Stromal cells had similar MCP-1 and CCR2 expression profile （68.17 % vs 4.27 %. P〈0.05）.Tumor cells of MCP-1 /CCR2 expression rates were 3.25 % and 32.76 %. Compared MCP-1 / CCR2 expression of stromal cells and tumor cells with different stages of disease, the activated stage and the stable stage had similar level （68.71% and 32.76 % vs 70.12 % and 53.39 %. P〉0.05）. Conclusion Most patients with MM bone marrow were expressed MCP-land CCR2. MCP-1 and CCR2 are the major MM cell surface expression of chemokine/receptor, which play important roles in the progress of.