摘要
目的观察单个核细胞趋化蛋白-1MCP-1及其受体CCR2在多发性骨髓瘤(MM)患者骨髓瘤细胞和骨髓基质细胞中的表达。方法经国内统-标准确诊为MM的患者15例,男7例,女8例,年龄38~67岁,平均年龄53.7岁。按照Durie—Salmon分期系统分期,I期2例、Ⅱ期5例、Ⅲ期8例。其他血液病患者10例为对照组。取样本进行骨髓瘤细胞与骨髓基质细胞的分离培养,在含13%胎牛血清的RPMI1640培养液中培养,每2d更换1次培养液,实验用细胞均处于对数生长期。采用流式细胞术检测骨髓瘤患者骨髓瘤细胞,骨髓基质细胞中MCP-1、CCR2水平。结果14例MM患者骨髓均表达MCP-1、CCR2,而对照组8例骨髓中趋化因子MCP-1、CCR2基本无表达。对照组和MM患者基质细胞上MCP-1、CCR2均阳性的表达率分别为4.27%和68.71%,两组比较差异具有统计学意义(P〈0.05)。瘤细胞上MCP-1、CCR2均阳性的表达率分别为3.25%和32.76%,两组比较差异具有统计学意义(P〈0.05)。处于疾病活动期的基质细胞和瘤细胞上MCP-1、CCR2均阳性的表达率分别为68.71%和32.76%,而处于疾病非活动期的的基质细胞和瘤细胞上MCP-1、CCR2均阳性的表达率分别为70.12%和53.39%,两组比较差异无统计学意义(P〉0.05)。结论大部分MM患者骨髓均表达MCP-1、CCR2。MCP-1及CCR2是MM细胞表面表达的主要趋化因子,对MM的疾病进展起着-个比较重要的作用。
Objective To observe the expression of chemokine (MCP-1) and chemokine receptor (CCR2) in bone marrow cells, bone marrow stromal cells of muhiple myeloma (MM) patients. Methods 15 cases were diagnosed by domestic uniform standard for MM patients, 7 cases of male, 8 cases of female, age range from 38 to 67 years, mean age 53.7 years old. According to the Durie-Salmon staging system, patients were divided into Ⅰ (2 cases), Ⅱ (5 cases) and m period(8 cases). Control group were from 10 cases of non-malignant blood disease patients. MCP-1, CCR2 expression were measured by flow cytometry. Results Almost 14 cases of bone marrow cells expressed MCP-1 and CCR2 in MM patients, while in the control group, bone marrow cells ahnost did not express MCP-1 and CCR2. Stromal cells had similar MCP-1 and CCR2 expression profile (68.17 % vs 4.27 %. P〈0.05).Tumor cells of MCP-1 /CCR2 expression rates were 3.25 % and 32.76 %. Compared MCP-1 / CCR2 expression of stromal cells and tumor cells with different stages of disease, the activated stage and the stable stage had similar level (68.71% and 32.76 % vs 70.12 % and 53.39 %. P〉0.05). Conclusion Most patients with MM bone marrow were expressed MCP-land CCR2. MCP-1 and CCR2 are the major MM cell surface expression of chemokine/receptor, which play important roles in the progress of.
出处
《白血病.淋巴瘤》
CAS
2012年第7期405-407,共3页
Journal of Leukemia & Lymphoma