IL-4和IFN-γ mRNA在子痫前期患者胎盘组织中的表达及意义

Expressions and Significance of IL-4 mRNA and IFN-γ mRNA in Placenta of Preeclampsia Patients

目的:检测胎盘组织中IFN-γ和IL-4的表达,探讨IFN-γ和IL-4在子痫前期的发病中的作用。方法:采用原位杂交法检测了20例正常妊娠孕妇和34例子痫前期组(包括16例轻度和18例重度)中的IFN-γ、IL-4 mRNA的表达水平,并通过图像分析系统对染色结果进行定量分析。结果:(1)IL-4 mRNA的表达在正常妊娠组、子痫前期轻度组和子痫前期重度组的表达无差异(P>0.05)。(2)与正常妊娠组、子痫前期轻度组相比,子痫前期重度组IFN-γmRNA的表达有差异性(P<0.05);子痫前期轻度组与正常妊娠组相比无差异(P>0.05)。(3)与正常妊娠组相比,子痫前期轻度组、重度组IFN-γmRNA/IL-4 mRNA的比值均有差异性(P<0.05),且随病情的加重比值增大。结论:Thl/Th2细胞因子的平衡偏离可能是导致子痫前期发病的病因之一。

Objective：To detect the expressions of IL-4 mRNA and IFN-γ mRNA in placenta of preeclampsia patients,and to explore the cause of preeclampsia.Methods：Patients with preeclampsia were chosen as the study group and normal pregnancy as and control group.The placental Th1-type cytokines IFN-γ mRNA and Th2-type cytokines IL-4 mRNA of the two groups are marked with in situ hybridization method,and the staining results were quantitatively detected and compared by color pathology image analysis system.Results：（1） The expressions of IL-4 mRNA were not statistically different between normal pregnancy group,mild preeclampsia group and severe preeclampsia group（P0.05）.（2） Compared with normal pregnancy group and mild pre-eclampsia group,the expression of IFN-γ mRNA was significantly different in severe pre-eclampsia group（P 0.05）;There was no difference between the mild preeclampsia group and normal pregnancy group（P0.05）.（3） Compared with normal pregnancy group,the IFN-γ mRNA/IL-4 mRNA ratio had difference in mild and severe preeclampsia group（P0.05）,and increased along with disease severity.Conclusion：The imbalance of Th1/Th2 cytokine may be one of the causes for preeclampsia.