寿胎丸提取液透过妊娠大鼠胎盘屏障的药物成分研究

Study on ingredients of Shou Tai Wan extract through placental barrier of rat in pregnancy

目的:对不同孕期妊娠大鼠给予寿胎丸提取液(STW),采用HPLC研究STW透过胎盘屏障的药物成分。方法:早、中、晚孕期Wistar大鼠灌胃STW,连续给药5天后,取母体血浆(母血)、脐静脉血及胚胎组织,用HPLC法检测其中所含药物成分。结果:在实验剂量下,各孕期1h母血中均检测到马钱子苷和槲皮苷,12h母血、脐静脉血及胚胎组织中均未检测到待测成分。结论:实验剂量下,STW的药物成分未透过胎盘屏障,提示妊娠期应用寿胎丸不会造成胎儿的宫内暴露。

Objective：Use HPLC to study the permeation of ingredients of Shou Tai Wan extract （STW） through placental barriers of Wistar rats at different stages of pregnancy. Methods ： The pregnant rats were administered by gavage STW for 5 days at early, middle and late stages of pregnancy. Hepatic portal vein plasma,umbilical veins plasma （at late stage of pregnancy） and embryonic tissues were obtained 1 h, 12 h after the final administration of STW. The compounds in biological specimens were identified by HPLC. Results：Among the range of experimental doses, loganin and quercitrin were main compounds in 1 h maternal blood but nothing could examined in 12 h maternal blood, umbilical veins plasma and embryonic tissues at different stages of pregnancy. Conclusion： Under the range of experimental doses,the compounds of STW didn＇ t permeate through placental barriers of rats. This result showed that the application of STW is safe to embryo in pregnancy.