黄芪药材的HPLC-DAD-ELSD指纹图谱研究

Study on Chromatography Fingerprint of Radix Astragali with Hyphenated Technique of HPLC-DAD-ELSD

目的:采用高效液相色谱法-二极管阵列检测器-蒸发光检测器(HPLC-DAD-ELSD)联用技术对黄芪药材进行指纹图谱研究。方法:采用Waters 2695-SpursilTMC18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水梯度洗脱,流速1.0 mL.min-1,柱温25℃,进样量20μL,检测波长254 nm。蒸发光散射检测器的条件为:漂移管温度112.8℃,载气流速3.2 L.min-1。结果:10批药材HPLC-DAD指纹图谱找到14个共有峰,鉴别了毛蕊异黄酮和芒柄花素;HPLC-ELSD指纹图谱找到9个共有峰,鉴别了毛蕊异黄酮苷、黄芪甲苷、黄芪皂苷Ⅲ、黄芪皂苷Ⅱ。结论:方法准确可靠,为全面控制黄芪药材质量提供了一种方法。

Objective： To research the chromatography fingerprint of Radix Astragali with hyphenated technique of HPLC-DAD-ELSD. Method： The chromatography conditions were defined as SpursilTM C18 column （4.6mm×250mm,5μm） ; mobile phase was A： ACN, B： H2O, gradient elution; flow speed was 1.0 mL ·min-1 ; temperature of column was set at 25 ℃; injection volume was 20 μL; detected wavelength was at 254 nm. The ELSD conditions were as follows ： the temperature of drift tube was 112.8 ℃ , the gas speed was 3.2 L .min- 1Result： In the chromatography fingerprint with HPLC-DAD of Radix Astragali, 14 common peaks were demarcated, calycosin and formononetin were identified; in the chromatography fingerprint with HPLC-ELSD of Radix Astragali, 9 common peaks were demarcated, calycosin-7-O-β-D-glucoside, astragaloside IV, astragaloside III and astragaloside II were identified. Conclusion： The method was accurate and reliable and the study may contribute in controlling of Radix Astragali.