穿山龙血清指纹图谱分析

Studies on Chromatography Fingerprint of Dioscorea nipponica Serum

目的:建立穿山龙大鼠体内HPLC血清指纹图谱。方法:应用Diamonsil C18柱(4.6 mm×250 mm,5μm),0.1%磷酸水-乙腈进行梯度洗脱,流速0.8 mL.min-1,检测波长210 nm,柱温25℃。结果:精密度、重复性、稳定性试验中共有峰相对峰面积、相对保留时间的RSD均<5.0%;结论:该方法简便、实用、可靠,为继续深入对穿山龙药效物质基础研究、药效作用机制研究等打下了坚实的基础。

Objective： To establish a method for Dioscorea nipponica rats serum HPLC fingerprinting. Method： The separation was performed on a Diamonsil Cls column （4.6mm×250mm,5μm） with 0. 1% phosphoric acid water and acetonitrile as mobile phase by gradient elution; the flow rate was 0. 8 mL·min-l; detection wavelength was set at 254 nm; the column temperature was maintained at 25℃. Result： The RSD of precision, reproducibility, stability test of relative peak area and relative retention time was less than 5%. Conclusion： The method is simple, practical, reliable, which would lay a solid foundation for further studies on pharmacodynamic material basis of Dioscorea nipponica and pharmacodynamic action mechanism research.