瓜蒌子的TLC鉴别和HPLC含量测定

TLC Identification and HPLC Assay on Trichosanthis Semen

目的:建立常用中药瓜蒌子的定性定量方法。方法:以栝楼仁二醇和α-菠菜甾醇为对照品,建立瓜蒌子的薄层色谱(TLC)鉴别技术;以栝楼仁二醇为对照品,建立高效液相色谱(HPLC)-紫外(PAD)检测的含量测定技术。结果:12份瓜蒌子的不皂化物,均可显示栝楼仁二醇和α-菠菜甾醇的薄层斑点;瓜蒌子中栝楼仁二醇的含量介于0.056 88%～0.087 28%。结论:栝楼仁二醇作为瓜蒌子的有效成分,可以作为瓜蒌子质量标准的一个重要指标。建议中药瓜蒌子中栝楼仁二醇的含量不得低于0.05%。

Objective： To establish the methods of qualitation and quantitation about Trichosanthis Semen, one of the commonly used Chinese material medica. Method： The thin-layer chromatography （TLC） method was found with the reference substance both karounidiol and α-spinasterol. The HPLC method was found with the PAD and the reference substance of karounidiol. Result： The unsaponifiable matter from all 12 Trichosanthis Semen samples showed the spots of both karounidiol and α-spinasterol on a TLC plate. The content of karounidiol in all 12 Trichosanthis Semen samples was 0. 056 88% -0. 087 28%. Conclusion： Karounidiol, one of the active components of Trichosanthis Semen, can be considered as an important index of the quality standard of Trichosanthis Semen. The content of karounidiol was suggested not less than 0.05% in Trichosanthis Semen.