不同品种灵芝主要活性成分分析

Analysis of the Main Active Ingredients from Different Varieties of Ganoderma lucidum

目的:对不同品种灵芝子实体中4类活性成分进行测定分析,为灵芝品种专用化育种提供依据。方法:采用紫外分光光度法测定灵芝样品总多糖、总三萜和蛋白质含量;采用反相高效液相色谱法测定灵芝样品麦角甾醇含量。结果:不同品种灵芝子实体中总多糖、总三萜、蛋白质均为含量较高的主要成分,但麦角甾醇的含量极低;4类成分在不同灵芝品种中含量差异显著,含量之间未发现有相关性。结论:灵芝多糖、三萜、蛋白质这3类各具典型功效的主要成分含量测定对灵芝子实体的质量控制均有重要的意义,并且可为制剂生产、临床应用的专用品种选育提供科学依据。

Objective： Four kinds of different active ingredients from the fruiting bodies from different varieties of Ganoderma lucidum have been detected. The experimental data could be used as the basis of the special medicinal varieties breeding. Method： The content of total polysaccharides, triterpenoid and protein was determined by UV spectrophotometer. Ergosterol was determined by HPLC. Result： Total polysaccharides, triterpenoid and protein： were the main components of different sources of Ganoderma lucidum. Ergosterol content was very low in the fruiting bodies. The content of the four ingredients was significantly different in different sources of G. lucidum. In addition, there were no correlations among the content of these four ingredients. Conclusion： Determination of polysaccharides, triterpenoid and protein was meaningful in the quality control of G. lucidum, and it can provide scientific basis for the breeding of special varieties.