云南美登木内生真菌Botryosphaeria sp．MHF次生代谢产物研究

Study on Secondary Metabolites of Endophytic Fungal Strain Botryosphaeria sp.MHF of Maytenus hookeri

目的:对云南美登木内生真菌Botryosphaeria sp.MHF的化学成分进行研究。方法:采用反相、正相等多种柱色谱法进行分离;应用波谱技术进行结构鉴定。结果:从云南美登木内生真菌B.sp.MHF的发酵物中分离得到8个化合物:分别是麦角甾-5-烯-3-醇(1)、麦角甾-4,6,8,22-四烯-3-酮(2)、麦角甾-3β,5α,9α-三羟基-7,22-二烯-6-酮(3)、麦角甾-7,22-二烯-3β,5α,6β-三醇(4)、麦角甾-5α,8α-环二氧-6,22-二烯-3-醇(5)、fusaproliferin(6)、脑苷脂C(7)和3,4,5-三羟基-四氢萘酮(8)。结论:所有化合物均为首次从以Murashige-Skoog培养基培养的该菌株中分离得到。

Objective： To study the secondary metabolites of endophytic fungal strain Botryosphaeria sp. MHF of Maytenus hookeri. Method： The chemical constituents were isolated by column chromatography such as normal phase or reverse phase etc. The structures were identified by spectroscopic analysis. Result： Eight compounds were obtained and elucidated as 22E, 24R-ergosta-5-en-3β-ol （1） , 22E, 24R-ergosta-4, 6, 8, 22- tetraen-3-one （2）, 22E, 24R-3/3, 5α, 9α-trihydroxy-ergosta-7, 22-diene-6-one （3）, 22E, 24R-ergosta-7, 22- diene-3/3, 5α, 6β-triol （4）, 22E, 24R-5α, 8α-epidioxyergosta-6, 22-dien-3β-ol （5）, fusaproliferin （6）, cerebroside C （7） and 3, 4, 5-trihydroxyl-tetralone （8）. Conclusion： All these compounds were isolated from this strain cultivated on Murashige-Skoog culture medium for the first time.