祛痰活血汤对非酒精性脂肪肝大鼠细胞凋亡的影响

Study on Non Alcoholic Fatty Liver by Qutan Huoxue Decoction

目的:建立非酒精性脂肪肝(NAFL)大鼠模型,观察非酒精性脂肪肝大鼠肝组织细胞凋亡程度,探讨祛痰活血汤治疗NAFL的作用机制。方法:取SD雄性大鼠60只,在实验条件下饲养1周后,随机分为2组:正常对照组(10只),给予普通饲料;造模组(50只),给予高脂饮食。在给予高脂饮食后的第2周开始,将造模组50只大鼠又分为模型对照组、祛痰活血汤低、中、高剂量治疗组(ig,4.5,9,13 g.kg-1)、凯西莱治疗组(ig,0.03 g.kg-1)。模型对照组、正常对照组不用任何药物,代之以同等剂量的生理盐水。实验动物自由进水和进食,每2周称重1次,并相应调整用药剂量。按照以上分组给药方案,第8,9,10周末,从造模组随机各抽取1～2只大鼠处死,观察肝组织病理变化以及血液生化指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、甘油三酯(TG)、总胆固醇(TC)等]。第10周处死所有大鼠,测定肝功能、血脂,取肝脏组织用10%甲醛溶液固定,石蜡包埋,连续切片,切片厚度为5μm,进行HE染色和TUNEL法检测细胞凋亡。结果:与正常对照组比较,模型对照组大鼠血清肝功能和血脂指标,肝组织病理炎症活动度、肝细胞凋亡表达指数均有明显的升高、加重(P<0.01);祛痰活血汤低、中、高剂量组、凯西莱组与模型对照组比,各项指标均能降低或减轻(P<0.05)。结论:祛痰活血汤可显著减轻NAFL大鼠肝组织脂肪变性程度,调节脂质代谢紊乱,一定程度上改善肝功能,降低组织细胞凋亡指数,这可能是其防治非酒精性脂肪肝病(NAFLD)的作用机制之一。

Objective： To observe apoptosis of nonalcoholic fatty liver in rat liver tissue and discuss mechanism of Qutan Huoxue decoction using non-alcoholic fatty liver rat model. Method： Sixty SD male rats were randomly divided into 2 groups： normal control group with 10 rats, which was given normal diet; model group with 50 rats, which was given high fat diet. The model group. After second week, 50 rats were divided into model control group, low dose group （Qutan Huoxue decoction 4.5 g·kg-1）, middle dose group （Qutan Huoxue decoction 9 g·kg-1）, high dose group （Qutan Huoxue decoction 13 g·kg-1） and Kaixilai drug group （30 mg ·kg-1）. Model control group and normal control group were given the same dose of NS. According to above drug plan, at the end of 8, 9, 10th week, 1-2 rats were dissected from the model group to observe pathological changes in liver tissue and blood biochemical parameters alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, triglycerides （TG）, total cholesterol （TC） and so on. All rats were dissected at the end of 10th week to observe hepatic function, blood lipid and apoptosis. Result： In model control group compared with normal control group, serum ALT, AST, TC, TG level, range of hepatic pathological inflammation, index in hepatic apoptosis showed significantly increased （P 〈 0.01 ） ; low, middle, high dose treatment groups of Qutan Huoxue decoction, Kaixilai treatment group compared with model group, their indexes were significantly decreased （P 〈 0.05 ）. Conclusion： Qutan Huoxue decoction can significantly reduce NAFL liver fatty degeneration, regulate lipid metabolism, improve liver function and decrease the apoptosis in liver.