复方人参健脑液对大鼠缺血再灌注脑细胞损伤的作用

Compound Ginseng Brain Tonic Liquid on Cerebral Ischemiareperfusion Injury in Rats

目的:观察复方人参健脑液对脑缺血再灌注损伤的保护作用。方法:大鼠随机分为正常对照组,脑缺血再灌注模型组,复方人参健脑液高剂量组(10 g.kg-1),复方人参健脑液低剂量(5 g.kg-1),维生素E组(1 g.kg-1)。采用颈动脉结扎法构建大鼠脑缺血再灌注损伤模型,硫代巴比妥酸法测定脑细胞脂质过氧化物含量,比色法测定还原型谷胱甘肽含量,氧电极法测定脑细胞耗氧量。结果:模型组大鼠脑脂质过氧化物含量明显增加(P<0.001),还原型谷胱甘肽含量减少(P<0.001),脑细胞耗氧量增加(P<0.01)。复方人参健脑液高剂量、低剂量组与模型组比较,脑脂质过氧化物水平降低(P<0.001),还原型谷胱甘肽含量增加(P<0.001),脑细胞耗氧量减少(P<0.001)。结论:复方人参健脑液对脑缺血再灌注损伤有一定的保护作用。

Objective： To study the effect of Compound Ginseng Brain Tonic Liquid （CGBTL） on cerebral ischemia-reperfusion injury. Method： Rats were randomly divided into normal control group, cerebral ischemia and reperfusion model group, large drug dose ＋ model group （ CGBTL 10 g·kg-1） , small drug dose ＋ model group （ CGBTL 5 g ·kg-1） , vitamin E ＋ model group. The model of cerebral ischemia reperfusion rats was established by ligaturing carotid. The lipid peroxide content of brain cell was measured with thiobarbituric acid. Reduced glutathione was determined by the method of spectrophotometer. Oxygen consumption was measured with clark oxygen electrode method. Result： In rats with cerebral ischemia-reperfusion injury, there showed an increase in lipid peroxide content （P〈 0. 001 ）, a decrease in reduced glutathione （P 〈 0. 001 ） and an increase in oxygen Consumption （P 〈0. 001 ） compared with those in the normal control group. In CGBTL large dose and small dose groups, compared with the model group, there showed a decrease in lipid peroxide content （P 〈 0. 001 ） , and an increase in reduced glutathione content （P 〈 0. 001 ） and a decrease in oxygen consumption （P 〈 0. 001 ）. In vitamin E treatment group, the outcomes were significant （P 〈 0. 001 ）. Conclusion ： CGBTL has preventive effect on cerebral ischemia-reperfusion injury.