摘要
目的:考察三叶青水提物及其含药血清对人胃腺癌细胞AGS体外增殖的影响。方法:清洁级小鼠随机分为5组:三叶青水提物高、中、低(20,10,5 g.kg-1)剂量组、环磷酰胺阳性对照(CTX,5 g.kg-1)组及阴性对照组(蒸馏水)。各组均ig给药,每次20 mL.kg-1,每天2次,连续给药3 d,最后1次给药后1 h断头取血,4℃静置4 h,3 000 r.min-1离心20 min,分离血清,56℃灭活30 min后,用0.22μm的滤膜过滤除菌,-20℃保存备用。对数生长期的AGS细胞经0.25%胰蛋白酶消化后,配成2.5×104/L的细胞悬液接种于96孔培养板,培养24 h后,加入不同浓度的三叶青水提物溶液及其含药血清,作用48 h后,采用MTT比色法在570 nm波长下测定A,观察不同浓度三叶青水提物及其含药血清对AGS细胞的抑制作用。结果:1~40 g.L-1三叶青水提物对AGS细胞有显著的抑制作用(P<0.01),IC50为13.15 g.L-1;三叶青水提物含药血清高、中、低(20,10,5 g.kg-1)剂量组也可显著地抑制AGS肿瘤细胞的增殖(P<0.01),IC50为70.06 g.L-1。二者均呈现出剂量依赖性。结论:三叶青水提物及其含药血清对AGS细胞均有较强抑制作用。
Objective: To investigate the effect of the water extract of Tetrastigmatis hemsleyani and the serum containing it on the proliferation in vitro. Method: The mice of clean grade were randomly assigned into 5 groups: high dose group (20 g·kg-1) , moderate dose group (10 g ·kg-1), low dose groups (5 g·kg-1), positive control cyclophosphamide (CTX, 5 g ·kg-1) group and negative control group (distilled water). Every group was administered by intragastric administration 20 mL·kg-1 each, twice a day within 3 successive days. One hour after the last administration on third day, blood was collected from every decapitated mouse. The blood was centrifugalized for 20 min at 3 000 r· min-1 after standing at 4 ℃. The separated serum was filtered to remove bacteria by 0.22 μm sepatate film at 56 ℃ after inactivated for 30 rain and stored at - 20 ℃ for later use. The suspensions of AGS cells in logarithmic growth phase digested by 0.25% tripsin were inoculated in 96 well culture plate with a cell density of 2.5 × 10^4/L. The different concentrations of the water extract of T. hemsleyani and the serum containing it were added into the culture after 24 h culture. MTT assay was used with determinating A value in the wavelength of 570 nm to evaluate the cell proliferation of the water extract of T. hemsleyani and the serum containing it for 48 h. Result: When the concentration of the water extract of T. hemsleyani was range from 1 g · L-1 to 40 g ·L-1 , a significant proliferation inhibiting (P 〈0.01 ) was shown and its 50% inhibition concentration (ICs0) was 13.15 g ·L-1. High, moderate and low dose treatment groups (20, 10, 5 g·kg-1) also significantly inhibited the proliferation of AGS cells and its ICs0was 70.06 g·L-1. Both of the proliferation inhibitings were shown in dose-dependent manner. Conclusion: The water extract of T. hemsleyani can strongly inhibit the AGS cells proliferation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期212-214,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省教育厅2010年度科研项目(Y201019049)
关键词
三叶青
AGS细胞
含药血清
体外增殖抑制作用
Tetrastigmatis hemsleyani
AGS cells
the serum containing T. hemsleyani
inhibition of proliferation in vitro