黄芪-当归配伍对气道变应性炎症大鼠IFN-γ,IL-4,STAT6,STAT4的影响

Effect of Astragali Radix and Angelicae Sinensis Radix on Contents of IFN-γ,IL-4,STAT6 and STAT4 of Airway Allergic Inflammation in Rats

目的:通过研究黄芪-当归配伍对气道变应性炎症模型大鼠血清干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)含量及肺组织信号转导因子和转录活化因子4(STAT4)、信号转导因子和转录活化因子6(STAT6)表达的影响,探讨黄芪-当归配伍治疗气道变应性炎症的作用机制。方法:采用卵清蛋白(OVA)致敏及喷雾激发的方法造成大鼠气道变应性炎症模型,分别用黄芪、当归、黄芪-当归配伍灌胃给药治疗。应用ELISA方法测定血清中IFN-γ、IL-4含量。应用Western blot方法检测肺组织STAT4、STAT6表达。结果:模型组血清IFN-γ含量降低,IL-4含量升高。黄芪组血清中IFN-γ含量增加,当归组血清中IL-4含量降低,芪归配伍组可增加血清中IFN-γ含量,降低IL-4含量。模型组肺组织STAT6、磷酸化信号转导因子和转录活化因子6(p-STAT6)表达增加,STAT4、磷酸化信号转导因子和转录活化因子4(p-STAT4)表达减少。黄芪组可降低STAT6,p-STAT6表达,增加p-STAT4表达。当归组可降低p-STAT6表达。芪归组可降低STAT6,p-STAT6,增加STAT4,p-STAT4表达。结论:黄芪-当归配伍可能通过抑制STAT6、促进STAT4,调节气道变应性炎症中Th1/Th2细胞因子平衡,从而对气道变应性炎症有抑制作用。

Objective： To observe the influence of Astragali Radix and Angelicae Sinensis Radix on level of interferon-γ （IFN-γ） and interleukin-4 （IL-4） in serum and expression of signal transducers and activators of transcription 6 （STAT6）, signal transducers and activators of transcription 4 （STAT4） of airway allergic inflammation in rats, in order to partly show the mechanism of Astragali Radix and Angelicae Sinensis Radix in treating airway allergic inflammation. Method： The model of airway allergic inflammation in rats were caused by OVA. Astragali Radix and Angelicae Sinensis Radix were used to control the model rats. The contents of IFN-γ and IL-4 in the serum were detected by ELISA. The expression of STAT6, STAT4 in lung tissue were detected by Western blot. Result： The level of IL-4 in serum of model group were significantly higher than those in the control group. The level of IFN-γ in serum of model group were significantly lower than those inthe control group. Astragali Radix can depress the level of IL-4 in serum, and increase the level of IFN-γ in serum. Angelica can depress the level of IL-4 in serum. Compatibility of Astragali Radix and Angelicae Sinensis Radix can depress the level of IL-4 in serum, and increase the level of IFN-γ in serum. Astragali Radix can depress the expression of STAT6, p- STAT6 in lung tissue, and increase the expression of p-STAT4. Angelicae Sinensis Radix can depress the expression of p-STAT6. Compatibility of Astragali Radix and Angelicae Sinensis Radix can depress the expression of STAT6, p- STAT6 in lung tissue, and increase the expression of STAT4, p-STAT4. Conclusion： Compatibility of Astragali Radix and Angelicae Sinensis Radix may adjust the balance of Thl and Th2 by restaining STAT6 and promoting STAT4 to control the airway allergic inflammation.