桑葚多糖抗疲劳作用及其机制

Anti-fatigue Effect and Mechanism of Mulberry Amylose Extracts in Mice

目的:探讨桑葚多糖抗疲劳作用及其机制。方法:雄性SPF级昆明种小鼠224只,随机分为4批,每批56只,分别进行负重游泳、血清尿胆素测定、血乳酸含量测定和肝糖原含量测定。每批动物再随机分为正常对照组、桑葚多糖高、中、低剂量组(900,300,100 mg.kg-1.d-1),连续ig 35 d后,分别测定游泳时间、血清尿胆素含量、血乳酸含量和肝糖原含量。结果:桑葚多糖高剂量组小鼠游泳时间(31.3±15.13)min较正常对照组(7.15±2.91)min明显延长;桑葚多糖高剂量组肝糖原含量(26.23±6.08)mg.g-1较正常对照组(18.75±3.12)mg.g-1明显增加,血清尿素氮含量(9.66±1.35)mmol.L-1较正常对照组(12.07±1.64)mmol.L-1明显减少(P<0.05)。结论:桑葚多糖具有抗疲劳作用。

Objective： To explore the anti-fatigue effect and mechanism of polysaccharides from mulberry amylose （MA）. Method： A total of 224 Kunming mice were randomly divided into 4 batches, each of which consisted of 4 groups： MA groups at the dosages of low （100 mg·kg-1）, middle （300 mg·kg-1） and high （900 mg·kg-1） , and the control group. Each mouse was administered once a day for 35 days. After the end of the administration, all the mice were evaluated for their weight-loaded swimming duration and the contents of hepatic glycongen, blood urea nitrogen （BUN） and blood lactic acid. Result： MA at the high dosages could prolong weight-loaded swimming duration （31.3 ± 15.13） min, compared with （7.15 ±2.91 ） min. In addition, an increase of hepatic glycongen （26.23 ± 6.08） mg·g-1, compared with （ 18.75 ± 3.12） mg ·g-1 and a decline of BUN （9.66 ± 1.35） mmol ·L-1, compared with （12.07 ± 1.64） mmol ·L-1 were observed in mince administered MA at the high dosages. Conclusion： MA could alleviate physical fatigue.