枳实消痞丸配合西药对幽门螺杆菌感染小鼠胃黏膜上皮细胞Fas蛋白表达的影响

Influence of Zhishi Xiaopi Pills Combined with Western Medicine on Fas Protein Expression in Gastric Epithelial Cells in Rats with Helicobacter Pylori Infection

目的:观察枳实消痞丸配合西药对幽门螺杆菌(Hp)感染小鼠胃黏膜上皮细胞Fas蛋白表达的影响,探讨中西药合用提高幽门螺杆菌感染相关性胃炎疗效的作用机制。方法:采用5%NaHCO3溶液及幽门螺杆菌国际标准菌株SS1灌喂BALB/c小鼠,建立Hp感染动物模型。对造模动物分别投以中药、西药和中西药联用,分别采用HE染色、TUNEL法和免疫组化(sABC法)观察胃黏膜损伤程度(LI)、细胞凋亡指数(AI)和Fas蛋白表达的变化。结果:3个治疗组均能抑制胃黏膜上皮细胞Fas蛋白的过高表达,模型组表达为(28.30±2.36)%,抑制程度中西药合用组>西药组>中药组[Fas表达依次为(15.50±2.39)%,(22.0±1.90)%,(25.90±2.34)%];3个治疗组对胃黏膜损伤有不同程度的改善,中西药合用组强于单用中药组、西药组[LI依次为(1.10±0.18),(1.80±0.25),(1.30±0.26)分];3个治疗组能对抗Hp所引起的细胞凋亡增加,模型组、中药组、西药组、中西药合用组AI依次为(16.60±1.74)%,(11.60±1.11)%,(8.70±0.88)%,(5.10±0.67)%,合用组抑制作用最强。结论:对细胞凋亡和胃黏膜上皮细胞Fas蛋白表达的抑制可能是枳实消痞丸合用西药三联提高幽门螺杆菌感染相关性胃炎疗效的机制之一。

Objective： To investigate the influence of Zhishi Xiaopi Pills combined with western medicine on Fas protein expression in gastric epithelial cells in rats with Helicobacter pylori infection and to explore the related mechanism. Method： BALB/c mice were fed with NaHCO3 solution of 5% and Helicobacter pylori （Hp） strains SS1 international standards to establish Hp infection, and randomly divided into three groups： the Chinese medicine group, the western medicine group, and the Chinese medicine combined with western medicine group. The injury of gastric mucous membrane, cell apoptosis index and epithelial cell expression of Fas were investigated by HE staining, Tunel method and immunohistochemical evaluation （SABC）. Result： In three groups, the over-expression of epithelial Fas protein could be inhibited by the interventions. The model group expression was （28.30 ± 2.36）%, while the Chinese medicine combined with western medicine group, the western medicine group and the Chinese medicine group were （15.50 ± 2.39）%, （22.0 ± 1.90）%, （25.9 ± 2. 34）% accordingly. The inhibition degree appeared to be the Chinese medicine combined with western medicine group 〉 the western medicine group 〉 the Chinese medicine group. In the three groups, the injury of gastric mucous membrane was improved to different degree. The Chinese medicine combined with western medicine group showed less injury （lesion index LI = 1.10 ± 0. 18） compared with the western medicine group （ 1.30 ± 0.26） and the Chinese medicine group （ 1.80 ± 0.25）. As cell apoptosis was concerned, the apoptosis index in the Chinese medicine combined with western medicine group （5.10 ± 0. 67）% was smaller than those in the western medicine group （8.70 ±0.88）% and the Chinese medicine group （11.60 ±1.11）%. The model showed an index of （16.60 ± 1.74）%. Conclusion： Zhishi Xiaopi Pills combined with western medicine can improve Helicobacter pylori-induced gastric injury. The meehanism may be related to inhibition of cell apoptosis and inhibition of expressions of Fas protein in gastric epithelial cells.